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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- 15614-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SF4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: LYEPNSQGY KYYRQKLEEF RKAKASSTGS FTAPDPGLKR KSPPEALSGS LPPATTCPAS STPAPTIIPA PAAPGKPASA ATVKRKRKSR WGPEEDKVEL PPAELVQRDV DASPSPLSVQ DLKGLGYEKG KPVGLVGVTE LSDAQKKQLK EQQEMQQMYD MIMQHKRAMQ DMQLLWEKAV QQHQHGYDSD EEVDSELGTW EHQLRRMEMD KTREWAEQLT KMGRGKHFIG DFLPPDELEK FMETFKALKE GREPDYSEYK EFKLTVENIG YQMLMKMGWK EGEGLGSEGQ GIKNPVNKGT TTVDGAGFGI DRPAELSKED DEYEAFRKRM MLAYRFRPNP LNNPRRPYY (198-645 aa encoded by BC002986)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.37 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 15614-1-AP (SF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse testis tissue were subjected to SDS PAGE followed by western blot with 15614-1-AP (SF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 15614-1-AP (SF4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( ) fixed HepG2 cells using 15614-1-AP (SF4 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IGG.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis using 15614-1-AP (SF4 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis using 15614-1-AP (SF4 antibody) at dilution of 1:100 (under 40x lens).
