Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-42503 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CC2D1A Recombinant Rabbit Monoclonal Antibody (JE55-34)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: MCF-7 cell lysate, A431 cell lysate, PC-12 cell lysate, human appendix tissue, human tonsil tissue, MCF-7. Subcellular Location: Centrosome, Nucleus, Cytoplasm.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JE55-34
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CC2D1A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. CC2D1A Recombinant Monoclonal Antibody (Product # MA5-42503) at 1:1,000 was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysate. Lane 2: A431 cell lysate. Lane 3: PC-12 cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of paraffin-embedded human appendix tissue using CC2D1A Recombinant Monoclonal Antibody (Product # MA5-42503). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the CC2D1A antibody at a dilution of 1:200 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of paraffin-embedded human tonsil tissue using CC2D1A Recombinant Monoclonal Antibody (Product # MA5-42503). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the CC2D1A antibody at a dilution of 1:50 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of CC2D1A in MCF-7 cells using CC2D1A Recombinant Monoclonal Antibody (Product # MA5-42503) at 1:50 (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1:1,000 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).