PA5-116460

antibody from Invitrogen Antibodies

Targeting: KCNIP1 KCHIP1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-116460

Provider

Invitrogen Antibodies

Product name

KCNIP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Positive controls: Human skin tissue lysates, rat brain tissue, SH-SY5Y.

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of KCNIP1 in human skin tissue using a KCNIP1 polyclonal antibody (Product #PA5-116460). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of KCNIP1 in a paraffin-embedded rat brain using a KCNIP1 polyclonal antibody (Product #PA5-116460). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of KCNIP1 in SH-SY5Y cells using a KCNIP1 polyclonal antibody (Product#PA5-116460). The cells were fixed, permeabilize, and stained with the primary antibody (1:50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1:1000 dilution for 30 minutes. The control was not incubated with the primary antibody (black).