14905-1-AP

antibody from Invitrogen Antibodies

Targeting: PSMC2 MSS1, Nbla10058, S7

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14905-1-AP

Provider

Invitrogen Antibodies

Product name

PSMC2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: KQTLQSEQP LQVARCTKII NADSEDPKYI INVKQFAKFV VDLSDQVAPT DIEEGMRVGV DRNKYQIHIP LPPKIDPTVT MMQVEEKPDV TYSDVGGCKE QIEKLREVVE TPLLHPERFV NLGIEPPKGV LLFGPPGTGK TLCARAVANR TDACFIRVIG SELVQKYVGE GARMVRELFE MARTKKACLI FFDEIDAIGG ARFDDGAGGD NEVQRTMLEL INQLDGFDPR GNIKVLMATN RPDTLDPALM RPGRLDRKIE FSLPDLEGRT HIFKIHARSM SVERDIRFEL LARLCPNSTG AEIRSVCTEA GMFAIRARRK IATEKDFLEA VNKVIKSYAK FSATPRYMTY N (84-433 aa encoded by BC002589)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 14905-1-AP (PSMC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse liver tissue were subjected to SDS PAGE followed by western blot with 14905-1-AP (PSMC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 14905-1-AP (PSMC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14905-1-AP (PSMC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells, using PSMC2 antibody 14905-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human ovary tumor using 14905-1-AP (PSMC2 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human ovary tumor using 14905-1-AP (PSMC2 antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 14905-1-AP (PSMC2 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 14905-1-AP (PSMC2 Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 14905-1-AP (PSMC2 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 14905-1-AP (PSMC2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PSMC2 (IP:14905-1-AP, 4ug; Detection:14905-1-AP 1:500) with mouse spleen tissue lysate 4800ug.