- HPA019080 - Atlas Antibodies CAPG antibody

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Supportive validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Independent antibody validation
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Experimental details: Western blot analysis using Anti-CAPG antibody HPA019080 (A) shows similar pattern to independent antibody HPA019092 (B).
Antibody #2 product nr: HPA019092
Antibody provider: Atlas Antibodies

Enhanced validation

Submitted by: 55af80e3e0991
Enhanced method: Genetic validation
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Experimental details: Confocal images of immunofluorescently stained human U-2 OS cells.The protein CAPG is shown in green. The image to the left show cells transfected with control siRNA and the image to the right show cells where CAPG has been downregulated with specific siRNA.
Sample type: U-2 OS cells
Primary Ab dilution: 1:54
Secondary Ab: Secondary Ab
Secondary Ab dilution: 1:800
Knockdown/Genetic Approaches Application: Immunocytochemistry
KD Reagent Type: siRNA
Downregulation: >50%
KD Reagent Provider: Thermo Fisher Scientific
KD Reagent Prod no: s2380
KD Reagent Prod page: KD Reagent Prod page
KD Reagent Prod no 2: s2381
KD Reagent Page 2: KD Reagent Page 2
KD Reagent Prod no 3: s228278
KD Reagent Page 3: KD Reagent Page 3
Antibody Lot Number: R08393
Appendix: 55b09c8cb50d1.pdf

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunofluorescent staining of human cell line A-431 shows localization to nucleus.
Sample type: HUMAN

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
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Experimental details: Immunohistochemistry analysis in human lung and liver tissues using HPA019080 antibody. Corresponding CAPG RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Independent antibody validation
Main image
Experimental details: Immunohistochemical staining of human lung, lymph node, skeletal muscle and small intestine using Anti-CAPG antibody HPA019080 (A) shows similar protein distribution across tissues to independent antibody HPA018843 (B).
Antibody #2 product nr: HPA018843
Antibody provider: Atlas Antibodies

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human lung shows strong cytoplasmic positivity in macrophages.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human liver shows no positivity in hepatocytes as expected.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human lung shows moderate to strong cytoplasmic positivity in macrophages.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human small intestine shows moderate to strong cytoplasmic positivity in cells in lamina propria.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human skeletal muscle shows no positivity in myocytes as expected.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human lymph node shows moderate to strong cytoplasmic positivity in macrophages.
Sample type: HUMAN

HPA019080