PA5-85568

antibody from Invitrogen Antibodies

Targeting: ARID1A B120, BAF250, BAF250a, C10rf4, C1orf4, P270, SMARCF1

Immunoprecipitation

Antibody data

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Validation data

Product number: PA5-85568 - Provider product page
Provider: Invitrogen Antibodies
Product name: ARID1A Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Keep as concentrated solution. Predicted reactivity: Mouse (81%), Bovine (84%). Positive Control: 293T, A431, HeLa, A549, HepG2, HepG2 nuclear extract. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.53 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

ARID1A protein structure - PA5-85568 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ARID1A in A) HepG2 whole cell extract, B) HepG2 nuclear extract using ARID1A polyclonal antibody (Product # PA5-85568) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ARID1A in whole cell extract using ARID1A polyclonal antibody (Product # PA5-85568) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ARID1A in whole cell extract using ARID1A polyclonal antibody (Product # PA5-85568) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ARID1A in various whole cell extracts using ARID1A polyclonal antibody (Product # PA5-85568) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of ARID1A was performed by separating 30 µg of (A) HepG2 and (B) HepG2 nuclear extract by 5 % SDS-PAGE. Proteins were transferred to a membrane and probed with a ARID1A Polyclonal Antibody (Product # PA5-85568) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using ARID1A Polyclonal Antibody (Product # PA5-85568). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID1A Polyclonal Antibody (Product # PA5-85568) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: ARID1A Polyclonal Antibody detects ARID1A protein by western blot analysis. Whole cell extracts (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with ARID1A Polyclonal Antibody (Product # PA5-85568) diluted by 1:1,000.