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antibody from Invitrogen Antibodies
Targeting: ARID1A
B120, BAF250, BAF250a, C10rf4, C1orf4, P270, SMARCF1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [7]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Other assay [2]
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- Product number
- PA5-85568 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ARID1A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution.
- Concentration
- 1.4 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ARID1A in A) HepG2 whole cell extract, B) HepG2 nuclear extract using ARID1A polyclonal antibody (Product # PA5-85568) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ARID1A in whole cell extract using ARID1A polyclonal antibody (Product # PA5-85568) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ARID1A in whole cell extract using ARID1A polyclonal antibody (Product # PA5-85568) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ARID1A in various whole cell extracts using ARID1A polyclonal antibody (Product # PA5-85568) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ARID1A was performed by separating 30 µg of (A) HepG2 and (B) HepG2 nuclear extract by 5 % SDS-PAGE. Proteins were transferred to a membrane and probed with a ARID1A Polyclonal Antibody (Product # PA5-85568) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ARID1A Polyclonal Antibody (Product # PA5-85568). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID1A Polyclonal Antibody (Product # PA5-85568) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ARID1A Polyclonal Antibody detects ARID1A protein by western blot analysis. Whole cell extracts (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with ARID1A Polyclonal Antibody (Product # PA5-85568) diluted by 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ARID1A Polyclonal Antibody detects ARID1A protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ARID1A protein stained by ARID1A Polyclonal Antibody (Product # PA5-85568) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ARID1A Polyclonal Antibody detects ARID1A protein at nucleus on human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. ARID1A Polyclonal Antibody (Product # PA5-85568) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ARID1A Polyclonal Antibody detects ARID1A protein at nucleus on human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. ARID1A Polyclonal Antibody (Product # PA5-85568) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ARID1A Polyclonal Antibody detects ARID1A protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. ARID1A stained by ARID1A Polyclonal Antibody (Product # PA5-85568) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of ARID1A in HepG2 whole cell extract, A) control with preimmune rabbit IgG, B) immunoprecipitation of ATG9A protein with ARID1A polyclonal antibody (Product # PA5-85568) using 4 µg of sample at a dilution of 1:500. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ARID1A Polyclonal Antibody immunoprecipitates ARID1A protein in IP experiments. IP samples: HepG2 whole cell extract. A. Control with 4 µg of preimmune Rabbit IgG. B. Immunoprecipitation of ARID1A protein by 4 µg ARID1A Polyclonal Antibody (Product # PA5-85568). 5 % SDS-PAGE. The immunoprecipitated ARID1A protein was detected by ARID1A Polyclonal Antibody (Product # PA5-85568) diluted at 1:500.
