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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PA5-32610 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TdT Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 500 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-TdT Recombinant Rabbit Monoclonal Antibody (RM379) (Product # PA5-32610) and a 58 kDa band corresponding to DNA nucleotidylexotransferase was observed across positive cell lines (Molt-4, Jurkat and Reh); and not across negative cell lines (A-431, Raji, Ramos, HeLa and Daudi). whole cell extracts (30 µg lysate) of MOLT-4 (Lane 1), A-431 (Lane 2), Jurkat (Lane 3), Raji (Lane 4), Ramos (Lane 5), Reh (Lane 6), HeLa (Lane 7) and Daudi (Lane 8) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product #WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TdT using anti-TdT Polyclonal Antibody (Product # PA5-32610) in Thymus Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:100.
