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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- GTX79245 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX79245, RRID:AB_11164204
- Product name
- Cyclophilin 40 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian, Rabbit, Simian
- Host
- Rabbit
Submitted references SLC25A26 overexpression impairs cell function via mtDNA hypermethylation and rewiring of methyl metabolism.
Menga A, Palmieri EM, Cianciulli A, Infantino V, Mazzone M, Scilimati A, Palmieri F, Castegna A, Iacobazzi V
The FEBS journal 2017 Mar;284(6):967-984
The FEBS journal 2017 Mar;284(6):967-984
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- By Western blot, this antibody detects a 40 kDa protein representing CyP-40 from rat brain extract.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on cancer biopsies of deparaffinized human Hepatocarcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a rabbit polyclonal antibody recognizing Cyclophilin D (GTX79245) or without primary antibody (negative control) overnight at 4¢XC in a humidified chamber.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on normal deparaffinized human Skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a rabbit polyclonal antibody recognizing Cyclophilin D (GTX79245) or without primary antibody (negative control) overnight at 4¢XC in a humidified chamber.
