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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- PA5-28376 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Carboxypeptidase E Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Carboxypeptidase H tansfected 293T cell, mouse brain.
- Concentration
- 0.68 mg/mL
Submitted references Why don't corticotroph tumors always produce Cushing's disease?
García-Martínez A, Cano DA, Flores-Martínez A, Gil J, Puig-Domingo M, Webb SM, Soto-Moreno A, Picó A
European journal of endocrinology 2019 Sep;181(3):351-361
European journal of endocrinology 2019 Sep;181(3):351-361
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CPE expression in transfected 293T cell line by CPE polyclonal antibody. Lane A: Non-transfected lysate. Lane B: CPE transfected lysate. 12% SDS PAGE. Carboxypeptidase E Polyclonal Antibody (Product # PA5-28376) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Carboxypeptidase E Polyclonal Antibody (Product # PA5-28376). Sample (20 µg of whole cell lysate). Lane A: mouse brain. 10% SDS PAGE. Carboxypeptidase E Polyclonal Antibody (Product # PA5-28376) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CPE expression in transfected 293T cell line by CPE polyclonal antibody. Lane A: Non-transfected lysate. Lane B: CPE transfected lysate. 12% SDS PAGE. Carboxypeptidase E Polyclonal Antibody (Product # PA5-28376) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Carboxypeptidase E Polyclonal Antibody detects Carboxypeptidase E protein at nucleus on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. Carboxypeptidase E Polyclonal Antibody (Product # PA5-28376) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
