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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [10]
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- Western blot [1]
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- Product number
- AF2199 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Legumain/Asparaginyl Endopeptidase Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Legumain/Asparaginyl Endopeptidase in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q99538- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references USP17 Suppresses Tumorigenesis and Tumor Growth through Deubiquitinating AEP.
Overexpression of asparaginyl endopeptidase is significant for esophageal carcinoma metastasis and predicts poor patient prognosis.
Suppression of asparaginyl endopeptidase attenuates breast cancer-induced bone pain through inhibition of neurotrophin receptors.
Glycosylation is important for legumain localization and processing to active forms but not for cystatin E/M inhibitory functions.
The Agaricus blazei-Based Mushroom Extract, Andosan™, Protects against Intestinal Tumorigenesis in the A/J Min/+ Mouse.
The asparaginyl endopeptidase legumain is essential for functional recovery after spinal cord injury in adult zebrafish.
Simvastatin inhibits glucose metabolism and legumain activity in human myotubes.
Differential secretome analysis reveals CST6 as a suppressor of breast cancer bone metastasis.
Cystatin E/M suppresses legumain activity and invasion of human melanoma.
Expression of the cysteine protease legumain in vascular lesions and functional implications in atherogenesis.
Chen X, Wang C, Liao K, Zhou S, Cao L, Chen J, Xu C, Lin Y
International journal of biological sciences 2019;15(4):738-748
International journal of biological sciences 2019;15(4):738-748
Overexpression of asparaginyl endopeptidase is significant for esophageal carcinoma metastasis and predicts poor patient prognosis.
Liu X, Wang Z, Zhang G, Zhu Q, Zeng H, Wang T, Gao F, Qi Z, Zhang J, Wang R
Oncology letters 2018 Jan;15(1):1229-1235
Oncology letters 2018 Jan;15(1):1229-1235
Suppression of asparaginyl endopeptidase attenuates breast cancer-induced bone pain through inhibition of neurotrophin receptors.
Yao P, Ding Y, Han Z, Mu Y, Hong T, Zhu Y, Li H
Molecular pain 2017 Jan-Dec;13:1744806917708127
Molecular pain 2017 Jan-Dec;13:1744806917708127
Glycosylation is important for legumain localization and processing to active forms but not for cystatin E/M inhibitory functions.
Lunde NN, Haugen MH, Bodin Larsen KB, Damgaard I, Pettersen SJ, Kasem R, Rut W, Drag M, Poreba M, Johansen HT, Solberg R
Biochimie 2017 Aug;139:27-37
Biochimie 2017 Aug;139:27-37
The Agaricus blazei-Based Mushroom Extract, Andosan™, Protects against Intestinal Tumorigenesis in the A/J Min/+ Mouse.
Hetland G, Eide DM, Tangen JM, Haugen MH, Mirlashari MR, Paulsen JE
PloS one 2016;11(12):e0167754
PloS one 2016;11(12):e0167754
The asparaginyl endopeptidase legumain is essential for functional recovery after spinal cord injury in adult zebrafish.
Ma L, Shen YQ, Khatri HP, Schachner M
PloS one 2014;9(4):e95098
PloS one 2014;9(4):e95098
Simvastatin inhibits glucose metabolism and legumain activity in human myotubes.
Smith R, Solberg R, Jacobsen LL, Voreland AL, Rustan AC, Thoresen GH, Johansen HT
PloS one 2014;9(1):e85721
PloS one 2014;9(1):e85721
Differential secretome analysis reveals CST6 as a suppressor of breast cancer bone metastasis.
Jin L, Zhang Y, Li H, Yao L, Fu D, Yao X, Xu LX, Hu X, Hu G
Cell research 2012 Sep;22(9):1356-73
Cell research 2012 Sep;22(9):1356-73
Cystatin E/M suppresses legumain activity and invasion of human melanoma.
Briggs JJ, Haugen MH, Johansen HT, Riker AI, Abrahamson M, Fodstad Ø, Maelandsmo GM, Solberg R
BMC cancer 2010 Jan 15;10:17
BMC cancer 2010 Jan 15;10:17
Expression of the cysteine protease legumain in vascular lesions and functional implications in atherogenesis.
Clerin V, Shih HH, Deng N, Hebert G, Resmini C, Shields KM, Feldman JL, Winkler A, Albert L, Maganti V, Wong A, Paulsen JE, Keith JC Jr, Vlasuk GP, Pittman DD
Atherosclerosis 2008 Nov;201(1):53-66
Atherosclerosis 2008 Nov;201(1):53-66
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Legumain/ Asparaginyl Endopeptidase by Western Blot. Western blot shows lysates of human kidney tissue, human heart tissue, and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Legumain/Asparaginyl Endopeptidase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2199) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Pro-Legumain/ Asparaginyl Endopeptidase at approximately 56 kDa and mature Legumain/Asparaginyl Endopeptidase 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
