14311-1-AP

antibody from Invitrogen Antibodies

Targeting: CASP10 FLICE-2, MCH4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14311-1-AP

Provider

Invitrogen Antibodies

Product name

Caspase 10/p23/17/p12 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: AIQIVTPPV DKEAESYQGE EELVSQTDVK TFLEALPQES WQNKHAGSNG NRATNGAPSL VSRGMQGASA NTLNSETSTK RAAVYRMNRN HRGLCVIVNN HSFTSLKDRQ GTHKDAEILS HVFQWLGFTV HIHNNVTKVE MEMVLQKQKC NPAHADGDCF VFCILTHGRF GAVYSSDEAL IPIREIMSHF TALQCPRLAE KPKLFFIQAC QGEEIQPSVS IEADALNPEQ APTSLQDSIP AEADFLLGLA TVPGYVSFRH VEEGSWYIQS LCNHLKKLVP RHEDILSILT AVNDDVSRRV DKQGTKKQMP QPAFTLRKKL VFPVPLDALS L (193-522 aa encoded by BC042844)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Apoptosised HeLa cells were subjected to SDS PAGE followed by western blot with 14311-1-AP (CASP10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 14311-1-AP (CASP10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using CASP10 antibody 14311-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using CASP10 antibody 14311-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human endometrial cancer using 14311-1-AP (CASP10 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human endometrial cancer using 14311-1-AP (CASP10 antibody) at dilution of 1:100 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-Caspase 10/p23/17/p12 (IP:14311-1-AP, 4ug; Detection:14311-1-AP 1:300) with HeLa cells lysate 3200ug.