PA5-29649

antibody from Invitrogen Antibodies

Targeting: CASP10 FLICE-2, MCH4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29649

Provider

Invitrogen Antibodies

Product name

Caspase 10 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: Jurkat, NCI-H929.

Concentration

1 mg/mL

References

Submitted references

Oxaliplatin resistance in colorectal cancer enhances TRAIL sensitivity via death receptor 4 upregulation and lipid raft localization.

Greenlee JD, Lopez-Cavestany M, Ortiz-Otero N, Liu K, Subramanian T, Cagir B, King MR
eLife 2021 Aug 3;10

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Caspase 10 Polyclonal Antibody (Product # PA5-29649). Sample (30 µg of whole cell lysate). Lane A: Jurkat. Lane B: NCI-H929 . 7.5% SDS PAGE. Caspase 10 Polyclonal Antibody (Product # PA5-29649) diluted at 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Caspase 10 in methanol-fixed HeLa cells using a Caspase 10 polyclonal antibody (Product # PA5-29649) at a 1:500 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of Caspase 10 was performed in paraffin-embedded human lung cancer tissue using Caspase 10 Polyclonal Antibody (Product # PA5-29649) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using Caspase 10 (Product # PA5-29649) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.