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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- AF6864 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Pro Caspase-10 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Pro Caspase-10 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Caspase-2, rhCaspase-3 (long and short forms), rhCaspase-3, rhCaspase-7, rhCaspase-8, rhCaspase-8 p10, rhCaspase-8 p20 and rhCaspase-9 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q92851- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Pro Caspase-10 by Western Blot. Western blot shows lysates of KG-1 human acute myelogenous leukemia cell line, human heart tissue, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Pro Caspase-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6864) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Pro Caspase-10 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Pro Caspase-10 in Jurkat Human Cell Line. Pro Caspase-10 was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Goat Anti-Human Pro Caspase-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6864) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
