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- Antibody Data
- Antigen structure
- References [2]
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- Western blot [1]
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- Product number
- MAB834 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Caspase-10 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human Caspase-10 in Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
AAC50644- Isotype
- IgG
- Antibody clone number
- 63131
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Identification of caspase-10 in human neutrophils and its role in spontaneous apoptosis.
Upregulation of the Fas receptor death-inducing signaling complex after traumatic brain injury in mice and humans.
Goepel F, Weinmann P, Schymeinsky J, Walzog B
Journal of leukocyte biology 2004 May;75(5):836-43
Journal of leukocyte biology 2004 May;75(5):836-43
Upregulation of the Fas receptor death-inducing signaling complex after traumatic brain injury in mice and humans.
Qiu J, Whalen MJ, Lowenstein P, Fiskum G, Fahy B, Darwish R, Aarabi B, Yuan J, Moskowitz MA
The Journal of neuroscience : the official journal of the Society for Neuroscience 2002 May 1;22(9):3504-11
The Journal of neuroscience : the official journal of the Society for Neuroscience 2002 May 1;22(9):3504-11
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human Caspase-10 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with Human FAS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF126) for for the indicated times. PVDF membrane was probed with 1 µg/mL of Human Caspase-10 Monoclonal Antibody (Catalog # MAB834), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Caspase-10 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
