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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- M02834 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-HMG4 Antibody Picoband™ (monoclonal, 7G13)
- Antibody type
- Monoclonal
- Description
- Mouse IgG monoclonal antibody for HMG4 detection. Tested with WB, IHC-P, ICC/IF, FCM in Human.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 7G13
- Vial size
- 100μg/vial
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of HMGB3 using anti- HMGB3 antibody (M02834). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Hela whole cell lysates, Lane 3: human T-47D whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human Caco-2 whole cell lysates. Lane 7: human SW620 whole cell lysates. Lane 8: human Raji whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- HMGB3 antigen affinity purified polyclonal antibody (Catalog # M02834) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HMGB3 at approximately 23KD. The expected band size for HMGB3 is at 23KD.
- Additional image
