Explore
Validate
LearnH00000826-M01
antibody from Novus Biologicals
Targeting: CAPNS1
30K, CANP, CANPS, CAPN4, CDPS
Western blot ELISA Immunocytochemistry Immunohistochemistry
Blocking/Neutralizing Proximity ligation assayAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00000826-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00000826-M01, RRID:AB_537367
- Product name
- Mouse Monoclonal Calpain small subunit 1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CAPNS1 - calpain, small subunit 1
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Genetic Models of Calpain Deficiency and Ectopic Expression.
FK506-binding protein 10 (FKBP10) regulates lung fibroblast migration via collagen VI synthesis.
Overexpression of a minimal domain of calpastatin suppresses IL-6 production and Th17 development via reduced NF-κB and increased STAT5 signals.
Control of basal autophagy by calpain1 mediated cleavage of ATG5.
The calpain inhibitor calpeptin prevents bleomycin-induced pulmonary fibrosis in mice.
Vitamin E and C supplementation does not ameliorate muscle dysfunction after anterior cruciate ligament surgery.
Gao Y, Hall C, MacLeod J, Greer PA
Methods in molecular biology (Clifton, N.J.) 2019;1915:261-274
Methods in molecular biology (Clifton, N.J.) 2019;1915:261-274
FK506-binding protein 10 (FKBP10) regulates lung fibroblast migration via collagen VI synthesis.
Knüppel L, Heinzelmann K, Lindner M, Hatz R, Behr J, Eickelberg O, Staab-Weijnitz CA
Respiratory research 2018 Apr 19;19(1):67
Respiratory research 2018 Apr 19;19(1):67
Overexpression of a minimal domain of calpastatin suppresses IL-6 production and Th17 development via reduced NF-κB and increased STAT5 signals.
Iguchi-Hashimoto M, Usui T, Yoshifuji H, Shimizu M, Kobayashi S, Ito Y, Murakami K, Shiomi A, Yukawa N, Kawabata D, Nojima T, Ohmura K, Fujii T, Mimori T
PloS one 2011;6(10):e27020
PloS one 2011;6(10):e27020
Control of basal autophagy by calpain1 mediated cleavage of ATG5.
Xia HG, Zhang L, Chen G, Zhang T, Liu J, Jin M, Ma X, Ma D, Yuan J
Autophagy 2010 Jan;6(1):61-6
Autophagy 2010 Jan;6(1):61-6
The calpain inhibitor calpeptin prevents bleomycin-induced pulmonary fibrosis in mice.
Tabata C, Tabata R, Nakano T
Clinical and experimental immunology 2010 Dec;162(3):560-7
Clinical and experimental immunology 2010 Dec;162(3):560-7
Vitamin E and C supplementation does not ameliorate muscle dysfunction after anterior cruciate ligament surgery.
Barker T, Leonard SW, Hansen J, Trawick RH, Ingram R, Burdett G, Lebold KM, Walker JA, Traber MG
Free radical biology & medicine 2009 Dec 1;47(11):1611-8
Free radical biology & medicine 2009 Dec 1;47(11):1611-8
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Calpain small subunit 1 Antibody (3C4) [H00000826-M01] - Analysis of CAPNS1 over-expressed 293 cell line, cotransfected with CAPNS1 Validated Chimera RNAi ( Cat # H00000826-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CAPNS1 monoclonal antibody (M01), clone 3C4 (Cat # H00000826-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Calpain small subunit 1 Antibody (3C4) [H00000826-M01] - Analysis of CAPNS1 expression in transfected 293T cell line by CAPNS1 monoclonal antibody (M01), clone 3C4.Lane 1: CAPNS1 transfected lysate(28.3 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Calpain small subunit 1 Antibody (3C4) [H00000826-M01] - CAPNS1 monoclonal antibody (M01), clone 3C4 Analysis of CAPNS1 expression in A-431.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Calpain small subunit 1 Antibody (3C4) [H00000826-M01] - Detection limit for recombinant GST tagged CAPNS1 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Calpain small subunit 1 Antibody (3C4) [H00000826-M01] - Analysis of monoclonal antibody to CAPNS1 on HeLa cell. Antibody concentration 20 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Calpain small subunit 1 Antibody (3C4) [H00000826-M01] - Analysis of monoclonal antibody to CAPNS1 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.
