Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-59446-100ul - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Calpain small subunit 1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Calpain small subunit 1 Antibody (1D11) [NBP2-59446] - Lane 1: Recombinant protein, Lane 2: HeLa cell lysates, Lane 3: A431 cell lysates, Lane 4: 293T cell lysates, Lane 5: Balb/3T3 cell lysates, Lane 6: U87MG cell lysates
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Calpain small subunit 1 Antibody (1D11) [NBP2-59446] - The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CAPNS1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: A431 cell lysate Lane 3.: 293T cell lysate