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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- 66127-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AK2 Monoclonal Antibody (3H2C10)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Immunogen sequence: MAPSVPAAE PEYPKGIRAV LLGPPGAGKG TQAPRLAENF CVCHLATGDM LRAMVASGSE LGKKLKATMD AGKLVSDEMV VELIEKNLET PLCKNGFLLD GFPRTVRQAE MLDDLMEKRK EKLDSVIEFS IPDSLLIRRI TGRLIHPKSG RSYHEEFNPP KEPMKDDITG EPLIRRSDDN EKALKIRLQA YHTQTTPLIE YYR (1-202 aa encoded by BC009405)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3H2C10
- Vial size
- 150 µL
- Concentration
- 1.8 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 66127-1-IG (AK2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 66127-1-Ig (AK2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using 66127-1-Ig (AK2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using 66127-1-IG (AK2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IGG.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using 66127-1-Ig (AK2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver slide using 66127-1-IG ( AK2 Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver slide using 66127-1-IG ( AK2 Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart slide using 66127-1-Ig (AK2 Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart slide using 66127-1-Ig (AK2 Antibody) at dilution of 1:50.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HepG2 cells were stained with .2ug AK2 antibody (66127-1-IG, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L) with dilution 1:1000.
