Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [5]
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Validation data
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- Product number
- PA5-52346 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- USP28 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: SCQMLLNQLR EITGIQDPSF LHEALKASNG DITQAVSLLT DERVKEPSQD TVATEPSEVE GSAANKEVLA KVIDLTHDNK DDLQAAIALS LLESPKIQAD GRDLNRMHEA TSAETKRSKR KRCEVWGENP NPND Highest antigen sequence identity to the following orthologs: Mouse - 89%, Rat - 89%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of USP28 in human cell line A-549 using a USP28 Polyclonal Antibody (Product # PA5-52346).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of USP28 in mouse cell line NIH-3T3 and rat cell line NBT-II using a USP28 Polyclonal Antibody (Product # PA5-52346).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of USP28 using USP28 Polyclonal Antibody (Product # PA5-52346) (A) shows similar pattern to an independent USP28 Polyclonal Antibody (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of USP28 in human cell line A-431 shows positivity in nucleus but excluded from the nucleoli. Samples were probed using an USP28 Polyclonal Antibody (Product # PA5-52346).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of USP28 in human cell line A-431 using an USP28 Polyclonal Antibody (Product # PA5-52346) shows localization to nucleoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of USP28 in human lymph node using USP28 Polyclonal Antibody (Product # PA5-52346).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of USP28 in human testis using USP28 Polyclonal Antibody (Product # PA5-52346).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of USP28 in human pancreas using USP28 Polyclonal Antibody (Product # PA5-52346).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of USP28 in human colon, lymph node, pancreas and testis using USP28 Polyclonal Antibody (Product # PA5-52346) (A) shows similar protein distribution across tissues to an independent USP28 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of USP28 in human colon using USP28 Polyclonal Antibody (Product # PA5-52346).