12296-1-AP

antibody from Invitrogen Antibodies

Targeting: SFXN1 FLJ12876, SLC56A1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12296-1-AP

Provider

Invitrogen Antibodies

Product name

SFXN1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MILIGRMSA QVPMNMTITG CMMTFYRTTP AVLFWQWINQ SFNAVVNYTN RSGDAPLTVN ELGTAYVSAT TGAVATALGL NALTKHVSPL IGRFVPFAAV AAANCINIPL MRQRELKVGI PVTDENGNRL GESANAAKQA ITQVVVSRIL MAAPGMAIPP FIMNTLEKKA FLKRFPWMSA PIQVGLVGFC LVFATPLCCA LFPQKSSMSV TSLEAELQAK IQESHPELRR VYFNKGL (87-322 aa encoded by BC020517)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.3 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PC-3 cells were subjected to SDS PAGE followed by western blot with 12296-1-AP (SFXN1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human kidney tissue were subjected to SDS PAGE followed by western blot with 12296-1-AP (SFXN1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 12296-1-AP (SFXN1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse liver tissue were subjected to SDS PAGE followed by western blot with 12296-1-AP (SFXN1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF7 cells were subjected to SDS PAGE followed by western blot with 12296-1-AP (SFXN1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney slide using 12296-1-AP (SFXN1 Antibody) at dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney slide using 12296-1-AP (SFXN1 Antibody) at dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using 12296-1-AP (SFXN1 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using (Product # 12296-1-AP) (SFXN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-SFXN1 (IP:12296-1-AP, 4ug; Detection:12296-1-AP 1:800) with mouse brain tissue lysate 4000ug.