Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA1-4517 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- P2Y2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Submitted references P2Y4 Nucleotide Receptor in Neuronal Precursors Induces Glutamatergic Subtype Markers in Their Descendant Neurons.
Uda Y, Xu S, Matsumura T, Takei Y
Stem cell reports 2016 Apr 12;6(4):474-482
Stem cell reports 2016 Apr 12;6(4):474-482
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of human brain lysate showing specific labeling of the ~50 kDa P2Y purinoceptor 2 protein.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of P2Y purinoceptor 2 in rat dorsal root ganglion samples using Product # PA1-4517.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Window of Nucleotide-Induced vGluT Expression (A) Differentiation of mouse ESCs was induced for 14 days on gelatin in the presence of 20 muM of the indicated nucleotides. Scale bar indicates 25 mum. (B) Cells positive for vGAT or vGluT in (A) were quantified as described in Experimental Procedures . The graph shows the mean of six independent experiments and the error bars indicate SE. * p < 0.05 relative to control (Student's t test). (C and E) Extracts were prepared from cells on the indicated differentiation days, and samples (20 mug of proteins) were analyzed by SDS-PAGE. (D) Intensities of signals in (C) were assessed with ImageJ version 1.45n. The graph shows the mean of three independent experiments and the error bars indicate SE. (F) ATP was added to the medium of differentiating mouse ESCs at a final concentration of 20 muM for the indicated days. (G) Cells positive for vGAT or vGluT in (F) were quantified as described in Experimental Procedures . The graph shows the mean of three independent experiments and the error bars indicate SE. * p < 0.05 relative to control (Student's t test). See also Figure S2 .