Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-27584 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TPH2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, U87-MG, SK-N-SH, IMR-32, SK-N-AS, Raw264.7, C2C12, mouse hippocampus, mouse midbrain. Predicted reactivity: Mouse (97%), Rat (99%), Zebrafish (91%), Japanese Medaka (90%), Dog (95%), Chicken (97%), Rhesus Monkey (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.3 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TPH2 using Various whole cell extracts (30 µg). Samples were loaded onto a 10% SDS-PAGE gel and probed with a TPH2 polyclonal antibody (Product # PA5-27584) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TPH2 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a TPH2 Polyclonal Antibody (Product # PA5-27584) at a dilution of 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TPH2 was performed by separating 50 µg of mouse tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a TPH2 Polyclonal Antibody (Product # PA5-27584) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TPH2 Polyclonal Antibody (Product # PA5-27584). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TPH2 Polyclonal Antibody (Product # PA5-27584) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TPH2 was performed by separating 50 µg of mouse tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a TPH2 Polyclonal Antibody (Product # PA5-27584) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TPH2 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a TPH2 Polyclonal Antibody (Product # PA5-27584) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of TPH2 was performed in DIV10 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: TPH2 Polyclonal Antibody (Product # PA5-27584) diluted at 1:500. Blue: Fluoroshield with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TPH2 Polyclonal Antibody detects TPH2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. TPH2 stained by TPH2 Polyclonal Antibody (Product # PA5-27584) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TPH2 Polyclonal Antibody detects TPH2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. TPH2 stained by TPH2 Polyclonal Antibody (Product # PA5-27584) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.