NB100-74555

antibody from Novus Biologicals

Targeting: TPH2 FLJ37295, NTPH

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-74555

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-74555, RRID:AB_1049988

Product name

Rabbit Polyclonal Tryptophan hydroxylase 2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat, Rabbit, Simian

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

An Adrenalectomy Mouse Model Reflecting Clinical Features for Chronic Fatigue Syndrome.

Lee JS, Jeon YJ, Park SY, Son CG
Biomolecules 2020 Jan 1;10(1)

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Mice lacking spinal α2GABA_A receptors: Altered GABAergic neurotransmission, diminished GABAergic antihyperalgesia, and potential compensatory mechanisms preventing a hyperalgesic phenotype.

Tudeau L, Acuña MA, Albisetti GW, Neumann E, Ralvenius WT, Scheurer L, Poe M, Cook JM, Johannssen HC, Zeilhofer HU
Brain research 2020 Aug 15;1741:146889

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Effects of neural estrogen receptor beta deletion on social and mood-related behaviors and underlying mechanisms in male mice.

Dombret C, Naulé L, Trouillet AC, Parmentier C, Hardin-Pouzet H, Mhaouty-Kodja S
Scientific reports 2020 Apr 10;10(1):6242

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Single-cell transcriptomes and whole-brain projections of serotonin neurons in the mouse dorsal and median raphe nuclei.

Ren J, Isakova A, Friedmann D, Zeng J, Grutzner SM, Pun A, Zhao GQ, Kolluru SS, Wang R, Lin R, Li P, Li A, Raymond JL, Luo Q, Luo M, Quake SR, Luo L
eLife 2019 Oct 24;8

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SSRIs target prefrontal to raphe circuits during development modulating synaptic connectivity and emotional behavior.

Soiza-Reilly M, Meye FJ, Olusakin J, Telley L, Petit E, Chen X, Mameli M, Jabaudon D, Sze JY, Gaspar P
Molecular psychiatry 2019 May;24(5):726-745

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Cav1.2 L-type calcium channels regulate stress coping behavior via serotonin neurons.

Ehlinger DG, Commons KG
Neuropharmacology 2019 Jan;144:282-290

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Identification of Embryonic Neural Plate Border Stem Cells and Their Generation by Direct Reprogramming from Adult Human Blood Cells.

Thier MC, Hommerding O, Panten J, Pinna R, García-González D, Berger T, Wörsdörfer P, Assenov Y, Scognamiglio R, Przybylla A, Kaschutnig P, Becker L, Milsom MD, Jauch A, Utikal J, Herrmann C, Monyer H, Edenhofer F, Trumpp A
Cell stem cell 2019 Jan 3;24(1):166-182.e13

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Rostral and Caudal Ventral Tegmental Area GABAergic Inputs to Different Dorsal Raphe Neurons Participate in Opioid Dependence.

Li Y, Li CY, Xi W, Jin S, Wu ZH, Jiang P, Dong P, He XB, Xu FQ, Duan S, Zhou YD, Li XM
Neuron 2019 Feb 20;101(4):748-761.e5

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Anatomically Defined and Functionally Distinct Dorsal Raphe Serotonin Sub-systems.

Ren J, Friedmann D, Xiong J, Liu CD, Ferguson BR, Weerakkody T, DeLoach KE, Ran C, Pun A, Sun Y, Weissbourd B, Neve RL, Huguenard J, Horowitz MA, Luo L
Cell 2018 Oct 4;175(2):472-487.e20

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Acute perturbation of Pet1-neuron activity in neonatal mice impairs cardiorespiratory homeostatic recovery.

Dosumu-Johnson RT, Cocoran AE, Chang Y, Nattie E, Dymecki SM
eLife 2018 Oct 23;7

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Prenatal and lactational bisphenol A exposure does not alter serotonergic neurons morphologically in the murine dorsal raphe nucleus.

Goto S, Ogi H, Fushiki S, Itoh K
Brain & development 2017 Jun;39(6):475-482

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Chronic-Stress-Induced Behavioral Changes Associated with Subregion-Selective Serotonin Cell Death in the Dorsal Raphe.

Natarajan R, Forrester L, Chiaia NL, Yamamoto BK
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Jun 28;37(26):6214-6223

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Xiaochaihutang attenuates depressive/anxiety-like behaviors of social isolation-reared mice by regulating monoaminergic system, neurogenesis and BDNF expression.

Ma J, Wang F, Yang J, Dong Y, Su G, Zhang K, Pan X, Ma P, Zhou T, Wu C
Journal of ethnopharmacology 2017 Aug 17;208:94-104

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Gene Expression Profiling with Cre-Conditional Pseudorabies Virus Reveals a Subset of Midbrain Neurons That Participate in Reward Circuitry.

Pomeranz LE, Ekstrand MI, Latcha KN, Smith GA, Enquist LW, Friedman JM
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Apr 12;37(15):4128-4144

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Ascending serotonin neuron diversity under two umbrellas.

Commons KG
Brain structure & function 2016 Sep;221(7):3347-60

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Altered tryptophan hydroxylase 2 expression in enteric serotonergic nerves in Hirschsprung's-associated enterocolitis.

Coyle D, Murphy JM, Doyle B, O'Donnell AM, Gillick J, Puri P
World journal of gastroenterology 2016 May 21;22(19):4662-72

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Reduced vesicular monoamine transport disrupts serotonin signaling but does not cause serotonergic degeneration.

Alter SP, Stout KA, Lohr KM, Taylor TN, Shepherd KR, Wang M, Guillot TS, Miller GW
Experimental neurology 2016 Jan;275 Pt 1(Pt 1):17-24

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Partial Raphe Dysfunction in Neurotransmission Is Sufficient to Increase Mortality after Anoxic Exposures in Mice at a Critical Period in Postnatal Development.

Barrett KT, Dosumu-Johnson RT, Daubenspeck JA, Brust RD, Kreouzis V, Kim JC, Li A, Dymecki SM, Nattie EE
The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 Apr 6;36(14):3943-53

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Differences in Anxiety-Like Behavior within a Batch of Wistar Rats Are Associated with Differences in Serotonergic Transmission, Enhanced by Acute SRI Administration, and Abolished By Serotonin Depletion.

Näslund J, Studer E, Pettersson R, Hagsäter M, Nilsson S, Nissbrandt H, Eriksson E
The international journal of neuropsychopharmacology 2015 Feb 25;18(8)

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Chronic L-dopa decreases serotonin neurons in a subregion of the dorsal raphe nucleus.

Stansley BJ, Yamamoto BK
The Journal of pharmacology and experimental therapeutics 2014 Nov;351(2):440-7

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Egr2-neurons control the adult respiratory response to hypercapnia.

Ray RS, Corcoran AE, Brust RD, Soriano LP, Nattie EE, Dymecki SM
Brain research 2013 May 20;1511:115-25

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The effects of glycogen synthase kinase-3beta in serotonin neurons.

Zhou W, Chen L, Paul J, Yang S, Li F, Sampson K, Woodgett JR, Beaulieu JM, Gamble KL, Li X
PloS one 2012;7(8):e43262

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Loss of deacetylation activity of Hdac6 affects emotional behavior in mice.

Fukada M, Hanai A, Nakayama A, Suzuki T, Miyata N, Rodriguiz RM, Wetsel WC, Yao TP, Kawaguchi Y
PloS one 2012;7(2):e30924

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Projections and interconnections of genetically defined serotonin neurons in mice.

Bang SJ, Jensen P, Dymecki SM, Commons KG
The European journal of neuroscience 2012 Jan;35(1):85-96

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Glutamatergic drive of the dorsal raphe nucleus.

Soiza-Reilly M, Commons KG
Journal of chemical neuroanatomy 2011 Jul;41(4):247-55

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Functional characterization of rhesus embryonic stem cell-derived serotonin neurons.

Tokuyama Y, Ingram SL, Woodward JS, Bethea CL
Experimental biology and medicine (Maywood, N.J.) 2010 May;235(5):649-57

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Chronic consumption of a high-fat diet during pregnancy causes perturbations in the serotonergic system and increased anxiety-like behavior in nonhuman primate offspring.

Sullivan EL, Grayson B, Takahashi D, Robertson N, Maier A, Bethea CL, Smith MS, Coleman K, Grove KL
The Journal of neuroscience : the official journal of the Society for Neuroscience 2010 Mar 10;30(10):3826-30

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Tryptophan hydroxylase 2 Antibody [NB100-74555] - Total protein from mouse brain, stomach and human brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Tryptophan Hydroxylase 2 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: Tryptophan hydroxylase 2 Antibody [NB100-74555] - Analysis of raphe nuclei isolated from Sprague-Dawley rat brains using TPH2 Polyclonal Antibody. PA1-778 was coupled to magnetic beads and added to raphe nuclei whole cell extracts for 15 minutes at RT. Immunoprecipitated proteins were assessed by Western blotting (WB), using either mouse anti-TPH2 (Ms TPH2, Lane 2) or sheep anti-TPH2 antibodies (Sh TPH2, Lane 3). As a positive control for the Western blot, non-immunoprecipitated brain lysates were probed with the mouse anti-TPH2 antibody (Lane 1). As a specificity control, a blot containing immunoprecipitated proteins was probed with an antibody specific for Tyrosine Hydroxylase (TH, Lane 4). Data courtesy of the Innovators Program.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody [NB100-74555] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse brain using Tryptophan hydroxylase 2 antibody at 1:25 dilution. The staining was developed using HRP-DAB detection method and the nuclei were counterstained with hematoxylin. The antibody generated staining of the neurons.

Other assay

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry Free-Floating: Tryptophan hydroxylase 2 Antibody [NB100-74555] - PFA-perfused mouse midbrain serotoninergic neurons. AlexaFluor488 used as secondary antibody. Neurons pseudocolored in yellow hot LUT. Image from verified customer review.