Antibody data
- Antibody Data
- Antigen structure
- References [35]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 11-7179-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-IL-17A Monoclonal Antibody (eBio64DEC17), FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBio64DEC17 antibody reacts with human IL-17A. The eBio64DEC17 antibody is a neutralizing antibody. Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells; the encoded protein exhibits 72 percent amino acid identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse IL-17A receptor. IL-23-dependent, IL-17A-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN gamma and IL-4, have each been found to negatively regulate the generation of these Th-17 cells. Intracellular staining by eBio64DEC17 antibody identifies the same cell population as the eBio64CAP17 antibody, as can be seen in co-staining experiments using both antibodies. Applications Reported: This eBio64DEC17 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This eBio64DEC17 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- eBio64DEC17
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references CXCL4 is a novel inducer of human Th17 cells and correlates with IL-17 and IL-22 in psoriatic arthritis.
Accumulation of T-helper 22 cells, interleukin-22 and myeloid-derived suppressor cells promotes gastric cancer progression in elderly patients.
Transcriptional signature of human pro-inflammatory TH17 cells identifies reduced IL10 gene expression in multiple sclerosis.
Cutting Edge: Selective Oral ROCK2 Inhibitor Reduces Clinical Scores in Patients with Psoriasis Vulgaris and Normalizes Skin Pathology via Concurrent Regulation of IL-17 and IL-10.
Berberine exerts an anti-inflammatory role in ocular Behcet's disease.
Single-cell profiling reveals GPCR heterogeneity and functional patterning during neuroinflammation.
Enhancing the toolbox to study IL-17A in cattle and sheep.
CD16+ Monocyte Subset Was Enriched and Functionally Exacerbated in Driving T-Cell Activation and B-Cell Response in Systemic Lupus Erythematosus.
Mycobacterium tuberculosis-Specific IL-21+IFN-γ+CD4+ T Cells Are Regulated by IL-12.
Safety and immunologic benefits of conversion to sirolimus in kidney transplant recipients with long-term exposure to calcineurin inhibitors.
Interaction among activated lymphocytes and mesenchymal cells through podoplanin is critical for a high IL-17 secretion.
Altered expression of miR-92a correlates with Th17 cell frequency in patients with primary biliary cirrhosis.
Increase of Th17 Cell Phenotype in Kidney Transplant Recipients with Chronic Allograft Dysfunction.
Interleukin-25 Mediates Transcriptional Control of PD-L1 via STAT3 in Multipotent Human Mesenchymal Stromal Cells (hMSCs) to Suppress Th17 Responses.
Improved Natural Killer cell activity and retained anti-tumor CD8(+) T cell responses contribute to the induction of a pathological complete response in HER2-positive breast cancer patients undergoing neoadjuvant chemotherapy.
Fiber composite slices for multiplexed immunoassays.
Mycobacterium bovis BCG Vaccination Induces Divergent Proinflammatory or Regulatory T Cell Responses in Adults.
Hyperreactive onchocerciasis is characterized by a combination of Th17-Th2 immune responses and reduced regulatory T cells.
A role for the TIM-3/GAL-9/BAT3 pathway in determining the clinical phenotype of multiple sclerosis.
Increased T helper type 17 response to pathogen stimulation in patients with primary sclerosing cholangitis.
An ACT1 mutation selectively abolishes interleukin-17 responses in humans with chronic mucocutaneous candidiasis.
Patients with cystic fibrosis have inducible IL-17+IL-22+ memory cells in lung draining lymph nodes.
Dysregulation of Th17 cells during the early post-transplant period in patients under calcineurin inhibitor based immunosuppression.
Increased frequencies of Th22 cells as well as Th17 cells in the peripheral blood of patients with ankylosing spondylitis and rheumatoid arthritis.
Brefeldin A, but not monensin, enables flow cytometric detection of interleukin-4 within peripheral T cells responding to ex vivo stimulation with Chlamydia trachomatis.
Inhibition of SLPI ameliorates disease activity in experimental autoimmune encephalomyelitis.
Anticardiac myosin immunity and chronic allograft vasculopathy in heart transplant recipients.
Effects of human respiratory syncytial virus, metapneumovirus, parainfluenza virus 3 and influenza virus on CD4+ T cell activation by dendritic cells.
Impaired CD4 and CD8 effector function and decreased memory T cell populations in ICOS-deficient patients.
Interleukin-12 is the optimum cytokine to expand human Th17 cells in vitro.
A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.
Prolonged, granulocyte-macrophage colony-stimulating factor-dependent, neutrophil survival following rheumatoid synovial fibroblast activation by IL-17 and TNFalpha.
Interleukin 17-producing gamma delta T cells increased in patients with active pulmonary tuberculosis.
Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells.
Distinct regulation of interleukin-17 in human T helper lymphocytes.
Affandi AJ, Silva-Cardoso SC, Garcia S, Leijten EFA, van Kempen TS, Marut W, van Roon JAG, Radstake TRDJ
European journal of immunology 2018 Mar;48(3):522-531
European journal of immunology 2018 Mar;48(3):522-531
Accumulation of T-helper 22 cells, interleukin-22 and myeloid-derived suppressor cells promotes gastric cancer progression in elderly patients.
Chen X, Wang Y, Wang J, Wen J, Jia X, Wang X, Zhang H
Oncology letters 2018 Jul;16(1):253-261
Oncology letters 2018 Jul;16(1):253-261
Transcriptional signature of human pro-inflammatory TH17 cells identifies reduced IL10 gene expression in multiple sclerosis.
Hu D, Notarbartolo S, Croonenborghs T, Patel B, Cialic R, Yang TH, Aschenbrenner D, Andersson KM, Gattorno M, Pham M, Kivisakk P, Pierre IV, Lee Y, Kiani K, Bokarewa M, Tjon E, Pochet N, Sallusto F, Kuchroo VK, Weiner HL
Nature communications 2017 Nov 17;8(1):1600
Nature communications 2017 Nov 17;8(1):1600
Cutting Edge: Selective Oral ROCK2 Inhibitor Reduces Clinical Scores in Patients with Psoriasis Vulgaris and Normalizes Skin Pathology via Concurrent Regulation of IL-17 and IL-10.
Zanin-Zhorov A, Weiss JM, Trzeciak A, Chen W, Zhang J, Nyuydzefe MS, Arencibia C, Polimera S, Schueller O, Fuentes-Duculan J, Bonifacio KM, Kunjravia N, Cueto I, Soung J, Fleischmann RM, Kivitz A, Lebwohl M, Nunez M, Woodson J, Smith SL, West RF, Berger M, Krueger JG, Ryan JL, Waksal SD
Journal of immunology (Baltimore, Md. : 1950) 2017 May 15;198(10):3809-3814
Journal of immunology (Baltimore, Md. : 1950) 2017 May 15;198(10):3809-3814
Berberine exerts an anti-inflammatory role in ocular Behcet's disease.
Yang Y, Wang Q, Xie M, Liu P, Qi X, Liu X, Li Z
Molecular medicine reports 2017 Jan;15(1):97-102
Molecular medicine reports 2017 Jan;15(1):97-102
Single-cell profiling reveals GPCR heterogeneity and functional patterning during neuroinflammation.
Tischner D, Grimm M, Kaur H, Staudenraus D, Carvalho J, Looso M, Günther S, Wanke F, Moos S, Siller N, Breuer J, Schwab N, Zipp F, Waisman A, Kurschus FC, Offermanns S, Wettschureck N
JCI insight 2017 Aug 3;2(15)
JCI insight 2017 Aug 3;2(15)
Enhancing the toolbox to study IL-17A in cattle and sheep.
Wattegedera SR, Corripio-Miyar Y, Pang Y, Frew D, McNeilly TN, Palarea-Albaladejo J, McInnes CJ, Hope JC, Glass EJ, Entrican G
Veterinary research 2017 Apr 8;48(1):20
Veterinary research 2017 Apr 8;48(1):20
CD16+ Monocyte Subset Was Enriched and Functionally Exacerbated in Driving T-Cell Activation and B-Cell Response in Systemic Lupus Erythematosus.
Zhu H, Hu F, Sun X, Zhang X, Zhu L, Liu X, Li X, Xu L, Shi L, Gan Y, Su Y
Frontiers in immunology 2016;7:512
Frontiers in immunology 2016;7:512
Mycobacterium tuberculosis-Specific IL-21+IFN-γ+CD4+ T Cells Are Regulated by IL-12.
Li L, Jiang Y, Lao S, Yang B, Yu S, Zhang Y, Wu C
PloS one 2016;11(1):e0147356
PloS one 2016;11(1):e0147356
Safety and immunologic benefits of conversion to sirolimus in kidney transplant recipients with long-term exposure to calcineurin inhibitors.
Yu JH, Kim KW, Kim BM, Chung BH, Cho ML, Choi BS, Park CW, Kim YS, Yang CW
The Korean journal of internal medicine 2016 May;31(3):552-9
The Korean journal of internal medicine 2016 May;31(3):552-9
Interaction among activated lymphocytes and mesenchymal cells through podoplanin is critical for a high IL-17 secretion.
Noack M, Ndongo-Thiam N, Miossec P
Arthritis research & therapy 2016 Jun 23;18:148
Arthritis research & therapy 2016 Jun 23;18:148
Altered expression of miR-92a correlates with Th17 cell frequency in patients with primary biliary cirrhosis.
Liang DY, Hou YQ, Luo LJ, Ao L
International journal of molecular medicine 2016 Jul;38(1):131-8
International journal of molecular medicine 2016 Jul;38(1):131-8
Increase of Th17 Cell Phenotype in Kidney Transplant Recipients with Chronic Allograft Dysfunction.
Chung BH, Kim KW, Kim BM, Doh KC, Cho ML, Yang CW
PloS one 2015;10(12):e0145258
PloS one 2015;10(12):e0145258
Interleukin-25 Mediates Transcriptional Control of PD-L1 via STAT3 in Multipotent Human Mesenchymal Stromal Cells (hMSCs) to Suppress Th17 Responses.
Wang WB, Yen ML, Liu KJ, Hsu PJ, Lin MH, Chen PM, Sudhir PR, Chen CH, Chen CH, Sytwu HK, Yen BL
Stem cell reports 2015 Sep 8;5(3):392-404
Stem cell reports 2015 Sep 8;5(3):392-404
Improved Natural Killer cell activity and retained anti-tumor CD8(+) T cell responses contribute to the induction of a pathological complete response in HER2-positive breast cancer patients undergoing neoadjuvant chemotherapy.
Muraro E, Comaro E, Talamini R, Turchet E, Miolo G, Scalone S, Militello L, Lombardi D, Spazzapan S, Perin T, Massarut S, Crivellari D, Dolcetti R, Martorelli D
Journal of translational medicine 2015 Jun 27;13:204
Journal of translational medicine 2015 Jun 27;13:204
Fiber composite slices for multiplexed immunoassays.
Kim J, Bae S, Song S, Chung K, Kwon S
Biomicrofluidics 2015 Jul;9(4):044109
Biomicrofluidics 2015 Jul;9(4):044109
Mycobacterium bovis BCG Vaccination Induces Divergent Proinflammatory or Regulatory T Cell Responses in Adults.
Boer MC, Prins C, van Meijgaarden KE, van Dissel JT, Ottenhoff TH, Joosten SA
Clinical and vaccine immunology : CVI 2015 Jul;22(7):778-88
Clinical and vaccine immunology : CVI 2015 Jul;22(7):778-88
Hyperreactive onchocerciasis is characterized by a combination of Th17-Th2 immune responses and reduced regulatory T cells.
Katawa G, Layland LE, Debrah AY, von Horn C, Batsa L, Kwarteng A, Arriens S, W Taylor D, Specht S, Hoerauf A, Adjobimey T
PLoS neglected tropical diseases 2015 Jan;9(1):e3414
PLoS neglected tropical diseases 2015 Jan;9(1):e3414
A role for the TIM-3/GAL-9/BAT3 pathway in determining the clinical phenotype of multiple sclerosis.
Saresella M, Piancone F, Marventano I, La Rosa F, Tortorella P, Caputo D, Rovaris M, Clerici M
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 Nov;28(11):5000-9
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 Nov;28(11):5000-9
Increased T helper type 17 response to pathogen stimulation in patients with primary sclerosing cholangitis.
Katt J, Schwinge D, Schoknecht T, Quaas A, Sobottka I, Burandt E, Becker C, Neurath MF, Lohse AW, Herkel J, Schramm C
Hepatology (Baltimore, Md.) 2013 Sep;58(3):1084-93
Hepatology (Baltimore, Md.) 2013 Sep;58(3):1084-93
An ACT1 mutation selectively abolishes interleukin-17 responses in humans with chronic mucocutaneous candidiasis.
Boisson B, Wang C, Pedergnana V, Wu L, Cypowyj S, Rybojad M, Belkadi A, Picard C, Abel L, Fieschi C, Puel A, Li X, Casanova JL
Immunity 2013 Oct 17;39(4):676-86
Immunity 2013 Oct 17;39(4):676-86
Patients with cystic fibrosis have inducible IL-17+IL-22+ memory cells in lung draining lymph nodes.
Chan YR, Chen K, Duncan SR, Lathrop KL, Latoche JD, Logar AJ, Pociask DA, Wahlberg BJ, Ray P, Ray A, Pilewski JM, Kolls JK
The Journal of allergy and clinical immunology 2013 Apr;131(4):1117-29, 1129.e1-5
The Journal of allergy and clinical immunology 2013 Apr;131(4):1117-29, 1129.e1-5
Dysregulation of Th17 cells during the early post-transplant period in patients under calcineurin inhibitor based immunosuppression.
Chung BH, Kim KW, Kim BM, Piao SG, Lim SW, Choi BS, Park CW, Kim YS, Cho ML, Yang CW
PloS one 2012;7(7):e42011
PloS one 2012;7(7):e42011
Increased frequencies of Th22 cells as well as Th17 cells in the peripheral blood of patients with ankylosing spondylitis and rheumatoid arthritis.
Zhang L, Li YG, Li YH, Qi L, Liu XG, Yuan CZ, Hu NW, Ma DX, Li ZF, Yang Q, Li W, Li JM
PloS one 2012;7(4):e31000
PloS one 2012;7(4):e31000
Brefeldin A, but not monensin, enables flow cytometric detection of interleukin-4 within peripheral T cells responding to ex vivo stimulation with Chlamydia trachomatis.
Vicetti Miguel RD, Maryak SA, Cherpes TL
Journal of immunological methods 2012 Oct 31;384(1-2):191-5
Journal of immunological methods 2012 Oct 31;384(1-2):191-5
Inhibition of SLPI ameliorates disease activity in experimental autoimmune encephalomyelitis.
Müller AM, Jun E, Conlon H, Sadiq SA
BMC neuroscience 2012 Mar 21;13:30
BMC neuroscience 2012 Mar 21;13:30
Anticardiac myosin immunity and chronic allograft vasculopathy in heart transplant recipients.
Kalache S, Dinavahi R, Pinney S, Mehrotra A, Cunningham MW, Heeger PS
Journal of immunology (Baltimore, Md. : 1950) 2011 Jul 15;187(2):1023-30
Journal of immunology (Baltimore, Md. : 1950) 2011 Jul 15;187(2):1023-30
Effects of human respiratory syncytial virus, metapneumovirus, parainfluenza virus 3 and influenza virus on CD4+ T cell activation by dendritic cells.
Le Nouën C, Hillyer P, Munir S, Winter CC, McCarty T, Bukreyev A, Collins PL, Rabin RL, Buchholz UJ
PloS one 2010 Nov 29;5(11):e15017
PloS one 2010 Nov 29;5(11):e15017
Impaired CD4 and CD8 effector function and decreased memory T cell populations in ICOS-deficient patients.
Takahashi N, Matsumoto K, Saito H, Nanki T, Miyasaka N, Kobata T, Azuma M, Lee SK, Mizutani S, Morio T
Journal of immunology (Baltimore, Md. : 1950) 2009 May 1;182(9):5515-27
Journal of immunology (Baltimore, Md. : 1950) 2009 May 1;182(9):5515-27
Interleukin-12 is the optimum cytokine to expand human Th17 cells in vitro.
Nady S, Ignatz-Hoover J, Shata MT
Clinical and vaccine immunology : CVI 2009 Jun;16(6):798-805
Clinical and vaccine immunology : CVI 2009 Jun;16(6):798-805
A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.
Kap YS, van Meurs M, van Driel N, Koopman G, Melief MJ, Brok HP, Laman JD, 't Hart BA
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2009 Dec;57(12):1159-67
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2009 Dec;57(12):1159-67
Prolonged, granulocyte-macrophage colony-stimulating factor-dependent, neutrophil survival following rheumatoid synovial fibroblast activation by IL-17 and TNFalpha.
Parsonage G, Filer A, Bik M, Hardie D, Lax S, Howlett K, Church LD, Raza K, Wong SH, Trebilcock E, Scheel-Toellner D, Salmon M, Lord JM, Buckley CD
Arthritis research & therapy 2008;10(2):R47
Arthritis research & therapy 2008;10(2):R47
Interleukin 17-producing gamma delta T cells increased in patients with active pulmonary tuberculosis.
Peng MY, Wang ZH, Yao CY, Jiang LN, Jin QL, Wang J, Li BQ
Cellular & molecular immunology 2008 Jun;5(3):203-8
Cellular & molecular immunology 2008 Jun;5(3):203-8
Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells.
Acosta-Rodriguez EV, Napolitani G, Lanzavecchia A, Sallusto F
Nature immunology 2007 Sep;8(9):942-9
Nature immunology 2007 Sep;8(9):942-9
Distinct regulation of interleukin-17 in human T helper lymphocytes.
Chen Z, Tato CM, Muul L, Laurence A, O'Shea JJ
Arthritis and rheumatism 2007 Sep;56(9):2936-46
Arthritis and rheumatism 2007 Sep;56(9):2936-46
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CD4-enriched human peripheral blood cells were polarized under Th17 conditions (with Human IL-23 Recombinant Protein (Product # 14-8239-63) for 10 days. Cells were restimulated with Protein Transport Inhibitor Cocktail (Product # 00-4980-03) (left) or Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right) for 6 hours. Cells were stained Anti-Human CD4 PE (Product # 12-0047-42) and Anti-Human IL-17A FITC using the Fixation & Permeabilization Buffers (Product # 88-8824-00). Viable cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis.
- Conjugate
- Green dye