Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-117656 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- USP10 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- This antibody has been tested in direct-ELISA
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 2.93 mg/mL
- Storage
- -20°C or -80°C if preferred
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of USP10 using USP10 Polyclonal Antibody (Product # PA5-117656) at 3 µg/mL. Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, Rat liver tissue. Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 88, 93 kDa. Observed band size: 88 kDa
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of USP10 in HeLa cells using USP10 Polyclonal Antibody (Product # PA5-117656) at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immnohistochemistry (Paraffin) analysis of USP10 in paraffin-embedded human testis tissue using USP10 Polyclonal Antibody (Product # PA5-117656) diluted at 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitating USP10 in A549 whole cell lysate. Lane 1: Rabbit control IgG A549 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1:2,000). Lane 2: USP10 Polyclonal Antibody (Product # PA5-117656) (8 µg) + A549 whole cell lysate (500 µg). Lane 3: A549 whole cell lysate (10 µg).
