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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- ARG63332 - Provider product page
- Provider
- Arigo
- Product name
- anti-FOXC2 antibody
- Antibody type
- Polyclonal
- Antigen
- RHAAPYSYDCTKY
- Description
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: HEK293 lysate (10ug protein in RIPA buffer) over expressing Human FOXC2 with DYKDDDDK tag stained with ARG63332 anti-FOXC2 antibody (0.1ug/ml) in Lane A overexpressing HEK293 cell lysate standed with anti- DYKDDDDK Tag (1/1000) in lane C. Mock-transfected HEK293 stained with ARG63332 anti-FOXC2 antibody (1mg/ml) in Lane B. Primary incubations were for 1 hour.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin embedded Human Breast cancer. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63332 anti-FOXC2 antibody at 2 µg/ml dilution followed by HRP-staining.
