H00127534-M01

antibody from Abnova Corporation

Targeting: GJB4 CX30.3

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00127534-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00127534-M01, RRID:AB_426129

Product name

GJB4 monoclonal antibody (M01), clone 1E3-1C12

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant GJB4.

Antigen sequence

MNWAFLQGLLSGVNKYSTVLSRIWLSVVFIFRVLV
YVVAAEEVWDDEQKDFVCNTKQPGCPNVCYDEFFP
VSHVRLWALQLILVTCPSLLVVMHVAYREERERKH
HLKHGPNAPSLYDNLSKKRGGLWWTYLLSLIFKAA
VDAGFLYIFHRLYKDYDMPRVVACSVEPCPHTVDC
YISRPTEKKVFTYFMVTTAAICILLNLSEVFYLVG
KRCMEIFGPRHRRPRCRECLPDTCPPYVLSQGGHP
EDGNSVLMKAGSAPVDAGGYP


Isotype

IgG

Antibody clone number

1E3-1C12

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

GJB4 monoclonal antibody (M01), clone 1E3-1C12 Western Blot analysis of GJB4 expression in Hela ( Cat # L013V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of GJB4 expression in transfected 293T cell line by GJB4 monoclonal antibody (M01), clone 1E3-1C12.Lane 1: GJB4 transfected lysate(30 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged GJB4 is approximately 3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol