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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunoprecipitation [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- NB100-77286 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-77286, RRID:AB_1085949
- Product name
- Rabbit Polyclonal Isopeptidase T/USP5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Isopeptidase T/USP5 Antibody [NB100-77286] - Detection of Human USP5 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-USP5 antibody Nb100-77286 used for IP at 3 ug/mg lysate. USP5 was also immunoprecipitated by rabbit anti-USP5 antibody NB100-77285, which recognizes an upstream epitope. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Isopeptidase T/USP5 Antibody [NB100-77286] - FFPE section of human colon carcinoma. Affinity purified rabbit anti-USP5 used at a dilution of 1:100. Detection: DAB staining using Immunohistochemistry Accessory Kit.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Isopeptidase T/USP5 Antibody [NB100-77286] - FFPE section of human ovarian carcinoma. Affinity purified rabbit anti-USP5used at a dilution of 1:500. Detection: DAB staining using Immunohistochemistry Accessory Kit
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Isopeptidase T/USP5 Antibody [NB100-77286] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- USP5/IsoT used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Isopeptidase T/USP5 Antibody [NB100-77286] - FFPE section of human colon carcinoma. Affinity purified rabbit anti-USP5 used at a dilution of 1:100. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1:100.
