Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-70397 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DLD Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This target displays homology in the following species: Cow: 100%; Dog: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 86%; Rabbit: 100%; Rat: 93%; Yeast: 86%; Zebrafish: 93%
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references SARS-CoV-2 infection rewires host cell metabolism and is potentially susceptible to mTORC1 inhibition.
Mullen PJ, Garcia G Jr, Purkayastha A, Matulionis N, Schmid EW, Momcilovic M, Sen C, Langerman J, Ramaiah A, Shackelford DB, Damoiseaux R, French SW, Plath K, Gomperts BN, Arumugaswami V, Christofk HR
Nature communications 2021 Mar 25;12(1):1876
Nature communications 2021 Mar 25;12(1):1876
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DLD on human Jurkat cells. The sample was probed with a DLD polyclonal antibody (Product # PA5-70397) using a primary antibody dilution of 0.2-1.0 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of DLD in paraffin-embedded human pineal tissue. Sample was probed with a DLD polyclonal antibody (Product # PA5-70397) at a dilution of 1:100. Detection was performed with a donkey anti-Rabbit Cy3 secondary antibody at a dilution of 1:200. Images were taken at 20x magnification with an exposure time of 0.5-2.0 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 1 SARS-CoV-2 infection alters TCA cycle metabolism. a Glutamine entry into the TCA cycle is reduced in SARS-CoV-2 infected cells. Mass isotopomer analysis of TCA cycle metabolites in mock or SARS-CoV-2 infected Vero kidney epithelial cells after 24 h incubation with U- 13 C-glutamine. Each isotopomer is a different color, which is defined in the figure. P values compare the total fraction labeled between mock and SARS-CoV-2 infected cells ( n = 3 biologically independent samples). b The oxidative TCA cycle is decreased in SARS-CoV-2 infected (red bars) Vero cells after 24 h infection compared with mock infected (gray bars) Vero cells ( n = 3 biologically independent samples). c The reductive TCA cycle is maintained in SARS-CoV-2 infected (red bars) Vero cells compared with mock infected (gray bars) Vero cells after 24 h infection ( n = 3 biologically independent samples). d Levels of alphaKGC members OGDH and DLD are reduced in SARS-CoV-2 infected Vero cells (representative of two biological independent samples). e Glucose-derived carbon entry into the TCA cycle is increased in SARS-CoV-2 infected cells. Mass isotopomer analysis of TCA cycle metabolites in mock or SARS-CoV-2 infected Vero kidney epithelial cells after 24 h incubation with U- 13 C-glucose. Each isotopomer is a different color, which is defined in the figure. P values compare the total fraction labeled between mock and SARS-CoV-2 infected cells ( n = 3 biologically independent samples). f Glucose-derived c