Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-27967 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-MOX1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, JC, BCL-1. Predicted reactivity: Mouse (84%), Rat (82%), Bovine (85%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.14 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Knockdown of angiopoietin like-2 protects against angiotensin II-induced cerebral endothelial dysfunction in mice.
Yu C, Luo X, Duquette N, Thorin-Trescases N, Thorin E
American journal of physiology. Heart and circulatory physiology 2015 Mar 1;308(5):H386-97
American journal of physiology. Heart and circulatory physiology 2015 Mar 1;308(5):H386-97
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Mox1 using 30 µg of A) 293T (B) A431 and C) HeLa lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Mox1 polyclonal antibody (Product # PA5-27967) at a dilution of 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Mox1 using 30 µg of A) JC and B) BCL-1 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Mox1 polyclonal antibody (Product # PA5-27967) at a dilution of 1:5000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Mox1 showing staining in the cytoplasm of HepG2 cells. HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a Mox1 polyclonal antibody (Product # PA5-27967) diluted at 1:500. Blue: Hoechst 33342 staining.