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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00055602-M04 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CARF Monoclonal Antibody (2E6)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Sequence of this protein is as follows: MAQEVSEYLS QNPRVAAWVE ALRCDGETDK HWRHRRDFLL RNAGDLAPAG GAASASTDEA ADAESGTRNR QLQQLISFSM AWANHVFLGC RYPQKVMDKI LSMAEGIKVT DAPTYTTRDE LVAKVKKRGI SSSNEGVEEP SKKRVIEGKN SSAVEQDHAK TSAKTERASA QQENSSTCIG SAIKSESGNS ARSSGISSQN SSTSDGDRSV SSQSSSSVSS QVTTAGSGKA SEAEAPDKHG SSFVSLLKSS VNSHMTQSTD SRQQSGSPKK SALEGSSASA SQSSSEIEVP LLGSSGSSEV ELPLLSSKPS SETASSGLTS KTSSEASVSS SVAKNSSSSG TSLLTPKSSS STNTSLLTSK STSQVAASLL ASKSSSQTSG SLVSKSTSLA SVSQLASKSS SQTSTSQLPS KSTSQSSESS VKFSCKLTNE DVKQKQPFFN RLYKTVAWKL VAVGGFSPNV NHGELLNAAI EALKATLDVF FVPLKELADL PQNKSSQESI VCELRCKSVY LGTGCGKSKE NAKAVASREA LKLFLKKKVV VKICKRKYRG SEIEDLVLLD EESRPVNLPP ALKHPQELL
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2E6
- Vial size
- 100 µg
- Concentration
- See Label
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CARF monoclonal antibody (M04), clone 2E6 Western Blot analysis of CARF expression in HeLa S3 NE (Cat # L013V3).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged CARF is approximately 1 ng/mL as a capture antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to CARF on HeLa cell. Antibody concentration is 25 µg/mL.
