Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [13]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 17491-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- USP20 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MGDSRDLCP HLDSIGEVTK EDLLLKSKGT CQSCGVTGPN LWACLQVACP YVGCGESFAD HSTIHAQAKK HNLTVNLTTF RLWCYACEKE VFLEQRLAAP LLGSSSKFSE QDSPPPSHPL KAVPIAVADE GESESEDDDL KPRGLTGMKN LGNSCYMNAA LQALSNCPPL TQFFLECGGL VRTDKKPALC KSYQKLVSEV WHKKRPSYVV PTSLSHGIKL VNPMFRGYAQ QDTQEFLRCL MDQLHEELKE PVVATVALTE ARDSDSSDTD EKREGDRSPS EDEFLSCDSS SDRGEGDGQG RGGGSSQAET ELLIPDEAGR AISEKERMKD RKFSWGQQRT NSEQVDEDAD VD (1-351 aa encoded by BC039593)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.25 mg/mL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 17491-1-AP (USP20 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of USP20 antibody (17491-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP20 transfected HeLa cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 17491-1-AP (USP20 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis using 17491-1-AP (USP20 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human ovary using 17491-1-AP (USP20 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis using 17491-1-AP (USP20 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain using 17491-1-AP (USP20 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain using 17491-1-AP (USP20 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human spleen using 17491-1-AP (USP20 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human spleen using 17491-1-AP (USP20 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung using 17491-1-AP (USP20 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung using 17491-1-AP (USP20 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human ovary using 17491-1-AP (USP20 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 17491-1-AP (USP20 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 17491-1-AP (USP20 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using (Product # 17491-1-AP) (USP20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-USP20 (IP:17491-1-AP, 4ug; Detection:17491-1-AP 1:500) with HeLa cells lysate 1200ug.
