BS4219

antibody from Bioworld Technology, Inc

Targeting: CASP6 MCH2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

BS4219

Provider

Bioworld Technology, Inc

Product name

Caspase 6 (phospho S257) polyclonal antibody

Antibody type

Polyclonal

Antigen

Synthetic phosphopeptide derived from human Caspase-6 around the phosphorylation site of Serine 257.

Description

Caspase-6 (Mch2) is one of the major executioner caspases functioning in cellular apoptotic processes. Upon apoptotic stimulation, initiator caspases such as caspase-9 are cleaved and activated. The activated upstream caspases further process downstream executioner caspases, such as caspase-3 and caspase-6, by cleaving them into large and small subunits, thereby initiating a caspase cascade leading to apoptosis. One of the major targets for caspase-6 is the membrane associated protein lamin A. The cleavage of this protein causes cell membrane malfunction, membrane blebbing and eventual cell death.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100ul

Concentration

1 mg/ml

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Bioworld Technology, Inc (provider)

Main image

Experimental details

Western blot (WB) analysis of p-Caspase 6 (S257) pAb at 1:500 dilutionLane1:MCF-7 cell lysate treated with colchicineLane2:Raw264.7 cell lysate treated with colchicineLane3:PC12 cell lysate treated with colchicine