MA5-32201

antibody from Invitrogen Antibodies

Targeting: CASP6 MCH2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-32201

Provider

Invitrogen Antibodies

Product name

Caspase 6 Recombinant Rabbit Monoclonal Antibody (SC56-09)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

SC56-09

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Caspase 6 in different lysates using a Monoclonal antibody (Product #MA5-32201) at a dilution of 1:1,000. Positive control: Lane 1: Mouse colon, Lane 2: MCF-7.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Caspase 6 Recombinant Rabbit Monoclonal Antibody (SC56-09) (Product # MA5-32201) and a 33 kDa band corresponding to Casp6 was observed across cell lines and tissues. Whole-cell extracts (30 µg lysate) of MCF7 (Lane 1), A-431 (Lane 2), OVCAR-3 (Lane 3), Jurkat (Lane 4), Jurkat treated with 50 µM Staurosporine for 8 hrs (Lane 5), Jurkat treated with 25 µM Etoposide for 1 hr (Lane 6), Mouse Colon (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX), 12 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Caspase 6 in SW480 cells using a Caspase 6 Monoclonal antibody (Product # MA5-32201) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Casp6 was performed using 70% confluent log phase OVCAR-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labelled with Caspase 6 Recombinant Rabbit Monoclonal Antibody (SC56-09) (Product # MA5-32201) at 1:100 dilution in 0.1% BSA, incubated at 4-degree celsius overnight and then labelled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790, 1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents SK-OV-3. Panel f represents control cells with no primary antibody to assess the background. The images were captured at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Caspase 6 of paraffin-embedded Human breast carcinoma tissue using a Caspase-6 Monoclonal antibody (Product #MA5-32201). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Caspase 6 of paraffin-embedded Human kidney tissue using a Caspase-6 Monoclonal antibody (Product #MA5-32201). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Caspase 6 of paraffin-embedded Mouse kidney tissue using a Caspase-6 Monoclonal antibody (Product #MA5-32201). Counter stained with hematoxylin.