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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 23206-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Claspin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MTGEVGSEV HLEINDPNVI SQEEADSPSD SGQGSYETIG PLSEGDSDEE IFVSKKLKNR KVLQDSDSET EDTNASPEKT TYDSAEEENK ENLYAGKNTK IKRIYKTVAD SDESYMEKSL YQENLEAQVK PCLELSLQSG NSTDFTTDRK SSKKHIHDKE GTAGKAKVKS KRRLEKEERK MEKIRQLKKK ETKNQEDDVE QPFNDSGCLL VDKDLFETGL EDENNSPLED EESLESIRAA VKNKVKKHKK KEPSLESGVH SFEEGSELSK GTTRKERKAA RLSKEALKQL HSETQRLIRE SALNLPYHMP ENKTIHDFFK RKPRPTCHGN AMALLKSSKY QSSHHKEIID TANTT (1-354 aa encoded by BC137279)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.29 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 23206-1-AP ( Claspin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using 23206-1-AP (Claspin antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IGG.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using 23206-1-AP (Claspin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-Claspin (IP:23206-1-AP, 5ug; Detection:23206-1-AP 1:1000) with HEK-293 cells lysate 2400ug.
