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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- NB100-248 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-248, RRID:AB_2082897
- Product name
- Rabbit Polyclonal Claspin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references The Replisome Mediates A-NHEJ Repair of Telomeres Lacking POT1-TPP1 Independently of MRN Function.
Claspin as a biomarker of human papillomavirus-related high grade lesions of uterine cervix.
Cdh1 regulates cell cycle through modulating the claspin/Chk1 and the Rb/E2F1 pathways.
Rai R, Gu P, Broton C, Kumar-Sinha C, Chen Y, Chang S
Cell reports 2019 Dec 10;29(11):3708-3725.e5
Cell reports 2019 Dec 10;29(11):3708-3725.e5
Claspin as a biomarker of human papillomavirus-related high grade lesions of uterine cervix.
Benevolo M, Musio A, Vocaturo A, Donà MG, Rollo F, Terrenato I, Carosi M, Pescarmona E, Vocaturo G, Mottolese M
Journal of translational medicine 2012 Jun 25;10:132
Journal of translational medicine 2012 Jun 25;10:132
Cdh1 regulates cell cycle through modulating the claspin/Chk1 and the Rb/E2F1 pathways.
Gao D, Inuzuka H, Korenjak M, Tseng A, Wu T, Wan L, Kirschner M, Dyson N, Wei W
Molecular biology of the cell 2009 Jul;20(14):3305-16
Molecular biology of the cell 2009 Jul;20(14):3305-16
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Claspin Antibody [NB100-248] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Affinity purified rabbit anti-Claspin antibody NB100-248 (lot number 2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. Claspin was also immunoprecipitated by a previous lot (lot number 1) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
