48-0428-42

antibody from Invitrogen Antibodies
Targeting: GP9 CD42a, GPIX
Provider product page for 48-0428-42

Antibody data

Product number
48-0428-42 - Provider product page
Provider
Invitrogen Antibodies
Product name
CD42a Monoclonal Antibody (GR-P), eFluor™ 450, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: This GR-P monoclonal antibody reacts with human CD42a, which is also known as glycoprotein IX (GPIX). CD42a is a 22-kDa transmembrane protein that is constitutively expressed on platelets and megakaryocytes. CD42a associates with CD42b, CD42c, and CD42d to form the GPIb-V-IX complex, which binds the von Willebrand factor, an endothelial adhesion protein, to mediate platelet adhesion and activation. This binding results in platelet aggregation at sites of vascular injury. This receptor complex has also been demonstrated to bind thrombin, factors XI and XII, P-selectin, and Mac-1. Finally, a point mutation within the leucine-rich motif of CD42a has been linked to Bernard-Soulier syndrome. Applications Reported: Anti-Human CD42a eFluor® 450 has been reported for use in flow cytometric analysis. Applications Tested: The GR-P antibody has been pre-titrated and tested by flow cytometric analysis of normal human platelets. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
GR-P
Vial size
100 Tests
Concentration
5 µL/Test
Storage
4° C, store in dark, DO NOT FREEZE!
GP9 protein structure - 48-0428-42 shown in red.