700180

antibody from Invitrogen Antibodies

Targeting: PSME3 Ki, PA28-gamma, PA28G, REG-GAMMA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

700180

Provider

Invitrogen Antibodies

Product name

PSME3 Recombinant Rabbit Monoclonal Antibody (20H9L19)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with canine, chicken, equine, orangutan, Xenopus and zebrafish based on sequence homology.

Antibody clone number

20H9L19

Concentration

0.5 mg/mL

References

Submitted references

Site-specific O-GlcNAcylation of Psme3 maintains mouse stem cell pluripotency by impairing P-body homeostasis.

Pecori F, Kondo N, Ogura C, Miura T, Kume M, Minamijima Y, Yamamoto K, Nishihara S
Cell reports 2021 Jul 13;36(2):109361

---

Mutant p53 (p53-R248Q) functions as an oncogene in promoting endometrial cancer by up-regulating REGγ.

Wang H, Bao W, Jiang F, Che Q, Chen Z, Wang F, Tong H, Dai C, He X, Liao Y, Liu B, Sun J, Wan X
Cancer letters 2015 May 1;360(2):269-79

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PSME3 in Jurkat (lane 1), HeLa (lane 2), rat liver (lane 3), PC12 (lane 4), and mouse liver (lane 5) using a PSME3 recombinant rabbit monoclonal antibody (Product # 700180) at a dilution of 1 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PSME3 in Jurkat (lane 1), HeLa (lane 2), rat liver (lane 3), PC12 (lane 4), and mouse liver (lane 5) using a PSME3 recombinant rabbit monoclonal antibody (Product # 700180) at a dilution of 1 µg/mL.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PSME3 in HeLa cells using a PSME3 recombinant rabbit monoclonal antibody (Product # 700180) at a dilution of 5 µg/mL in the absence of peptide (top) and presence of immunogenic peptide (bottom), followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody at a dilution of 1:1000. Actin was stained with Alexa Fluor 568 Phalloidin (Product # A12380). Hoechst only (blue, left), AF488 signal only (green, middle) and composite image with Phalloidin (right).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PSME3 in formalin-fixed, paraffin-embedded human thyroid (top left) and lung carcimona (top right) and Hodgkin lymphoma (bottom left) using a PSME3 monoclonal antibody (Product # 700180) at a dilution of 5 µg/mL. Staining was visualized using DAB and images were taken at a magnification of 20x. Results show strong nuclear staining in tumor cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PSME3 in formalin-fixed, paraffin-embedded human thyroid (top left) and lung carcimona (top right) and Hodgkin lymphoma (bottom left) using a PSME3 monoclonal antibody (Product # 700180) at a dilution of 5 µg/mL. Staining was visualized using DAB and images were taken at a magnification of 20x. Results show strong nuclear staining in tumor cells.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of PSME3 in Jurkat cells using a PSME3 recombinant rabbit monoclonal antibody (Product # 700180) at a dilution of 2ug. Cells were fixed and permeabilized using FIX & PERM (Product # GAS004) reagent, and detection was performed using an Alexa Fluor 488 goat anti-rabbit IgG (black) compared to a control without primary antibody (gray). Pre-incubation with the immunogenic peptide decreased the signal (red).