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- Antibody Data
- Antigen structure
- References [21]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-5621 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-5621, RRID:AB_2166848
- Product name
- Anti-PML
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
Submitted references Functional reorganization of promyelocytic leukemia nuclear bodies during BK virus infection.
SUMO E3 ligase activity of TRIM proteins.
Autophagy regulates myeloid cell differentiation by p62/SQSTM1-mediated degradation of PML-RARα oncoprotein.
Functional interaction of nuclear domain 10 and its components with cytomegalovirus after infections: cross-species host cells versus native cells.
Dual functions of ASCIZ in the DNA base damage response and pulmonary organogenesis.
Membrane permeable cyclic peptidyl inhibitors against human Peptidylprolyl Isomerase Pin1.
Gamma interferon-dependent transcriptional memory via relocalization of a gene locus to PML nuclear bodies.
SENP3-mediated de-conjugation of SUMO2/3 from promyelocytic leukemia is correlated with accelerated cell proliferation under mild oxidative stress.
HDAC3 as a molecular chaperone for shuttling phosphorylated TR2 to PML: a novel deacetylase activity-independent function of HDAC3.
ERG-associated protein with SET domain (ESET)-Oct4 interaction regulates pluripotency and represses the trophectoderm lineage.
The two functions of herpes simplex virus 1 ICP0, inhibition of silencing by the CoREST/REST/HDAC complex and degradation of PML, are executed in tandem.
Sin3B expression is required for cellular senescence and is up-regulated upon oncogenic stress.
Histone deacetylase 7 promotes PML sumoylation and is essential for PML nuclear body formation.
Transactivation of cellular genes involved in nucleotide metabolism by the regulatory IE1 protein of murine cytomegalovirus is not critical for viral replicative fitness in quiescent cells and host tissues.
Signal-dependent regulation of transcription by histone deacetylase 7 involves recruitment to promyelocytic leukemia protein nuclear bodies.
Retinoic acid-stimulated sequential phosphorylation, PML recruitment, and SUMOylation of nuclear receptor TR2 to suppress Oct4 expression.
A redundant oncogenic potential of the retinoic receptor (RAR) α, β and γ isoforms in acute promyelocytic leukemia
CD8 T cells control cytomegalovirus latency by epitope-specific sensing of transcriptional reactivation.
Arsenic trioxide affects signal transducer and activator of transcription proteins through alteration of protein tyrosine kinase phosphorylation.
Premature truncation of a novel protein, RD3, exhibiting subnuclear localization is associated with retinal degeneration.
Sp2 localizes to subnuclear foci associated with the nuclear matrix.
Jiang M, Entezami P, Gamez M, Stamminger T, Imperiale MJ
mBio 2011;2(1):e00281-10
mBio 2011;2(1):e00281-10
SUMO E3 ligase activity of TRIM proteins.
Chu Y, Yang X
Oncogene 2011 Mar 3;30(9):1108-16
Oncogene 2011 Mar 3;30(9):1108-16
Autophagy regulates myeloid cell differentiation by p62/SQSTM1-mediated degradation of PML-RARα oncoprotein.
Wang Z, Cao L, Kang R, Yang M, Liu L, Zhao Y, Yu Y, Xie M, Yin X, Livesey KM, Tang D
Autophagy 2011 Apr;7(4):401-11
Autophagy 2011 Apr;7(4):401-11
Functional interaction of nuclear domain 10 and its components with cytomegalovirus after infections: cross-species host cells versus native cells.
Cosme RC, Martínez FP, Tang Q
PloS one 2011 Apr 28;6(4):e19187
PloS one 2011 Apr 28;6(4):e19187
Dual functions of ASCIZ in the DNA base damage response and pulmonary organogenesis.
Jurado S, Smyth I, van Denderen B, Tenis N, Hammet A, Hewitt K, Ng JL, McNees CJ, Kozlov SV, Oka H, Kobayashi M, Conlan LA, Cole TJ, Yamamoto K, Taniguchi Y, Takeda S, Lavin MF, Heierhorst J
PLoS genetics 2010 Oct 21;6(10):e1001170
PLoS genetics 2010 Oct 21;6(10):e1001170
Membrane permeable cyclic peptidyl inhibitors against human Peptidylprolyl Isomerase Pin1.
Liu T, Liu Y, Kao HY, Pei D
Journal of medicinal chemistry 2010 Mar 25;53(6):2494-501
Journal of medicinal chemistry 2010 Mar 25;53(6):2494-501
Gamma interferon-dependent transcriptional memory via relocalization of a gene locus to PML nuclear bodies.
Gialitakis M, Arampatzi P, Makatounakis T, Papamatheakis J
Molecular and cellular biology 2010 Apr;30(8):2046-56
Molecular and cellular biology 2010 Apr;30(8):2046-56
SENP3-mediated de-conjugation of SUMO2/3 from promyelocytic leukemia is correlated with accelerated cell proliferation under mild oxidative stress.
Han Y, Huang C, Sun X, Xiang B, Wang M, Yeh ET, Chen Y, Li H, Shi G, Cang H, Sun Y, Wang J, Wang W, Gao F, Yi J
The Journal of biological chemistry 2010 Apr 23;285(17):12906-15
The Journal of biological chemistry 2010 Apr 23;285(17):12906-15
HDAC3 as a molecular chaperone for shuttling phosphorylated TR2 to PML: a novel deacetylase activity-independent function of HDAC3.
Gupta P, Ho PC, Ha SG, Lin YW, Wei LN
PloS one 2009;4(2):e4363
PloS one 2009;4(2):e4363
ERG-associated protein with SET domain (ESET)-Oct4 interaction regulates pluripotency and represses the trophectoderm lineage.
Yeap LS, Hayashi K, Surani MA
Epigenetics & chromatin 2009 Oct 7;2(1):12
Epigenetics & chromatin 2009 Oct 7;2(1):12
The two functions of herpes simplex virus 1 ICP0, inhibition of silencing by the CoREST/REST/HDAC complex and degradation of PML, are executed in tandem.
Gu H, Roizman B
Journal of virology 2009 Jan;83(1):181-7
Journal of virology 2009 Jan;83(1):181-7
Sin3B expression is required for cellular senescence and is up-regulated upon oncogenic stress.
Grandinetti KB, Jelinic P, DiMauro T, Pellegrino J, Fernández Rodríguez R, Finnerty PM, Ruoff R, Bardeesy N, Logan SK, David G
Cancer research 2009 Aug 15;69(16):6430-7
Cancer research 2009 Aug 15;69(16):6430-7
Histone deacetylase 7 promotes PML sumoylation and is essential for PML nuclear body formation.
Gao C, Ho CC, Reineke E, Lam M, Cheng X, Stanya KJ, Liu Y, Chakraborty S, Shih HM, Kao HY
Molecular and cellular biology 2008 Sep;28(18):5658-67
Molecular and cellular biology 2008 Sep;28(18):5658-67
Transactivation of cellular genes involved in nucleotide metabolism by the regulatory IE1 protein of murine cytomegalovirus is not critical for viral replicative fitness in quiescent cells and host tissues.
Wilhelmi V, Simon CO, Podlech J, Böhm V, Däubner T, Emde S, Strand D, Renzaho A, Lemmermann NA, Seckert CK, Reddehase MJ, Grzimek NK
Journal of virology 2008 Oct;82(20):9900-16
Journal of virology 2008 Oct;82(20):9900-16
Signal-dependent regulation of transcription by histone deacetylase 7 involves recruitment to promyelocytic leukemia protein nuclear bodies.
Gao C, Cheng X, Lam M, Liu Y, Liu Q, Chang KS, Kao HY
Molecular biology of the cell 2008 Jul;19(7):3020-7
Molecular biology of the cell 2008 Jul;19(7):3020-7
Retinoic acid-stimulated sequential phosphorylation, PML recruitment, and SUMOylation of nuclear receptor TR2 to suppress Oct4 expression.
Gupta P, Ho PC, Huq MM, Ha SG, Park SW, Khan AA, Tsai NP, Wei LN
Proceedings of the National Academy of Sciences of the United States of America 2008 Aug 12;105(32):11424-9
Proceedings of the National Academy of Sciences of the United States of America 2008 Aug 12;105(32):11424-9
A redundant oncogenic potential of the retinoic receptor (RAR) α, β and γ isoforms in acute promyelocytic leukemia
A Marinelli, D Bossi, P G Pelicci, S Minucci
Leukemia 2007 Jan;21(4):647-650
Leukemia 2007 Jan;21(4):647-650
CD8 T cells control cytomegalovirus latency by epitope-specific sensing of transcriptional reactivation.
Simon CO, Holtappels R, Tervo HM, Böhm V, Däubner T, Oehrlein-Karpi SA, Kühnapfel B, Renzaho A, Strand D, Podlech J, Reddehase MJ, Grzimek NK
Journal of virology 2006 Nov;80(21):10436-56
Journal of virology 2006 Nov;80(21):10436-56
Arsenic trioxide affects signal transducer and activator of transcription proteins through alteration of protein tyrosine kinase phosphorylation.
Wetzler M, Brady MT, Tracy E, Li ZR, Donohue KA, O'Loughlin KL, Cheng Y, Mortazavi A, McDonald AA, Kunapuli P, Wallace PK, Baer MR, Cowell JK, Baumann H
Clinical cancer research : an official journal of the American Association for Cancer Research 2006 Nov 15;12(22):6817-25
Clinical cancer research : an official journal of the American Association for Cancer Research 2006 Nov 15;12(22):6817-25
Premature truncation of a novel protein, RD3, exhibiting subnuclear localization is associated with retinal degeneration.
Friedman JS, Chang B, Kannabiran C, Chakarova C, Singh HP, Jalali S, Hawes NL, Branham K, Othman M, Filippova E, Thompson DA, Webster AR, Andréasson S, Jacobson SG, Bhattacharya SS, Heckenlively JR, Swaroop A
American journal of human genetics 2006 Dec;79(6):1059-70
American journal of human genetics 2006 Dec;79(6):1059-70
Sp2 localizes to subnuclear foci associated with the nuclear matrix.
Moorefield KS, Yin H, Nichols TD, Cathcart C, Simmons SO, Horowitz JM
Molecular biology of the cell 2006 Apr;17(4):1711-22
Molecular biology of the cell 2006 Apr;17(4):1711-22
No comments: Submit comment
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 219, 111, 83, 48, 32, 26, 17
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 48
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human oral mucosa shows distinct nuclear positivity in squamous epithelial cells.
- Validation comment
- Staining pattern consistent with experimental and/or bioinformatic data.
