Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- PAB4343 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4343, RRID:AB_1578914
- Product name
- PML polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PML.
- Antigen sequence
PRKVIKMESEEGKEA
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references ZIP kinase triggers apoptosis from nuclear PML oncogenic domains.
Leukemia-associated translocation products able to activate RAS modify PML and render cells sensitive to arsenic-induced apoptosis.
High frequency of alternative splicing of human genes participating in the HIV-1 life cycle: a model using TSG101, betaTrCP, PPIA, INI1, NAF1, and PML.
Kawai T, Akira S, Reed JC
Molecular and cellular biology 2003 Sep;23(17):6174-86
Molecular and cellular biology 2003 Sep;23(17):6174-86
Leukemia-associated translocation products able to activate RAS modify PML and render cells sensitive to arsenic-induced apoptosis.
Puccetti E, Beissert T, Güller S, Li JE, Hoelzer D, Ottmann OG, Ruthardt M
Oncogene 2003 Oct 9;22(44):6900-8
Oncogene 2003 Oct 9;22(44):6900-8
High frequency of alternative splicing of human genes participating in the HIV-1 life cycle: a model using TSG101, betaTrCP, PPIA, INI1, NAF1, and PML.
Favre M, Butticaz C, Stevenson B, Jongeneel CV, Telenti A
Journal of acquired immune deficiency syndromes (1999) 2003 Oct 1;34(2):127-33
Journal of acquired immune deficiency syndromes (1999) 2003 Oct 1;34(2):127-33
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PmL polyclonal antibody (Cat # PAB4343) in HeLa cell line lysates (35 ug/lane). PmL (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with PML polyclonal antibody (Cat # PAB4343) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)