PA5-28839

antibody from Invitrogen Antibodies

Targeting: TGFB1I1 ARA55, Hic-5, TSC-5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28839

Provider

Invitrogen Antibodies

Product name

HIC5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: Hic-5 tansfected 293T cell.

Concentration

0.79 mg/mL

References

Submitted references

GPR75 receptor mediates 20-HETE-signaling and metastatic features of androgen-insensitive prostate cancer cells.

Cárdenas S, Colombero C, Panelo L, Dakarapu R, Falck JR, Costas MA, Nowicki S
Biochimica et biophysica acta. Molecular and cell biology of lipids 2020 Feb;1865(2):158573

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Basement membrane ligands initiate distinct signalling networks to direct cell shape.

Randles MJ, Lausecker F, Humphries JD, Byron A, Clark SJ, Miner JH, Zent R, Humphries MJ, Lennon R
Matrix biology : journal of the International Society for Matrix Biology 2020 Aug;90:61-78

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of TGFB1I1 expression in transfected 293T cell line by TGFB1I1 polyclonal antibody. Lane A: Non-transfected lysate. Lane B: TGFB1I1 transfected lysate. 12% SDS PAGE. HIC5 Polyclonal Antibody (Product # PA5-28839) diluted at 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HIC5 in paraformaldehyde-fixed U2OS cells using a HIC5 polyclonal antibody (Product # PA5-28839) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.