HPA039061

antibody from Atlas Antibodies

Targeting: MAP6 FLJ41346, KIAA1878, MAP6-N, STOP

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA039061

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA039061, RRID:AB_2676325

Product name

Anti-MAP6

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

TSASHKPTRKAKDKQAVSGQAAKKKSAEGPSTTKP
DDKEQSKEMNNKLAEAKESLAQPVSDSSKTQGPVA
TEPDKDQGSVVPGLLKGQGPMVQEPLKKQGSVVPG
PPKDLGPM


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell line CACO-2 and human cell line HeLa.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Western blot analysis using Anti-MAP6 antibody HPA039061 (A) shows similar pattern to independent antibody HPA039062 (B).

Antibody #2 product nr

HPA039062

Antibody provider

Atlas Antibodies

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Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human cerebral cortex and pancreas tissues using Anti-MAP6 antibody. Corresponding MAP6 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebellum shows strong cytoplasmic and nuclear positivity in purkinje cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas shows low expression as expected.

Sample type

HUMAN