A303-562A

antibody from Invitrogen Antibodies

Targeting: PARN DAN

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A303-562A

Provider

Invitrogen Antibodies

Product name

PARN Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human PARN by western blot. Samples: Whole cell lysate (10 µg) from LNCaP, MCF-7, Hep-G2, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PARN antibody (Product # A303-562A lot 2) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse PARN by western blot. Samples: Whole cell lysate (10 µg) from CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PARN antibody (Product # A303-562A lot 2) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human PARN by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PARN antibody A303-562A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. PARN was also immunoprecipitated by rabbit anti-PARN antibody A303-561A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human PARN by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PARN (Product # A303-562A; lot 2) used at a dilution of 1:1,000 (1 µg/mL). Detection: DAB. Counterstain: Hematoxylin (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse PARN by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- PARN (Cat. No. A303-562A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human PARN by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- PARN (Cat. No. A303-562A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB. Counterstain: Hematoxylin (blue).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human PARN by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PARN antibody (Product # A303-562A lot 2) used for IP at 6 µg per reaction. PARN was also immunoprecipitated by a previous lot of this antibody (A303-562A lot 1) and a second antibody against a different epitope of PARN (Product # A303-561A). For blotting immunoprecipitated PARN (Product # A303-562A) was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.