GTX48709

antibody from GeneTex

Targeting: CENPU CENP-50, CENP-U, KLIP1, MLF1IP, PBIP1

Western blot (Enhanced validation)

ELISA (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

GTX48709

Provider

GeneTex

Proper citation

GeneTex Cat#GTX48709, RRID:AB_11164839

Product name

Mlf1 Interacting Protein antibody

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Western blot

Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot using GeneTex's affinity purified anti-MLF1IP / PBIP1 antibody shows detection of endogenous MLF1IP protein (a tier of four modified protein bands indicated by the arrowheads) in lysates of Hela cells (- lane). Cells treated with MLF1IP / PBIP1 shRNA (+ lane) show no staining. The identities of the higher and lower molecular weight bands are unknown. Primary antibody was used at 1:1,000.

KD Reagent Type

siRNA

Downregulation

>75%

KD Reagent Provider

Customized siRNA

KD Reagent Prod no

GTX48709

KD Reagent Prod page

http://www.genetex.com

Antibody Lot Number

39224

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Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot using GeneTex's affinity purified anti-MLF1IP / PBIP1 antibody shows detection of endogenous MLF1IP protein (a tier of four modified protein bands indicated by the arrowheads) in lysates of Hela cells (- lane). Cells treated with MLF1IP / PBIP1 shRNA (+ lane) show no staining. The identities of the higher and lower molecular weight bands are unknown. Primary antibody was used at 1:1,000. Personal Communication, K.S. Lee, NCI, Bethesda, MD.

Validation comment

WB