A07180

antibody from Boster Biological Technology

Targeting: CENPU CENP-50, CENP-U, KLIP1, MLF1IP, PBIP1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

A07180

Provider

Boster Biological Technology

Product name

Anti-MLF1 CENPU Antibody

Antibody type

Polyclonal

Description

Polyclonal antibody for PBIP1/CENPU detection. Host: Rabbit.Size: 100μL. Tested applications: IHC. Reactive species: Human. PBIP1/CENPU information: Molecular Weight: 47522 MW; Subcellular Localization: Cytoplasm. Nucleus. Chromosome, centromere, kinetochore. Localizes in the kinetochore domain of centromeres. Colocalizes with PLK1 at the interzone between the inner and the outer kinetochore plates; Tissue Specificity: Expressed at high levels in the testis, fetal liver, thymus, bone marrow and at lower levels in the lymph nodes, placenta, colon and spleen. Present in all cell lines examined, including B-cells, T-cells, epithelial cells and fibroblast cells. Expressed at high levels in glioblastoma cell lines.

Reactivity

Human

Host

Rabbit

Vial size

100μL

Concentration

0.88 mg/mL by UV absorbance at 280 nm

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of MLF1 expression in detection of endogenous MLF1IP protein (a tier of four modified protein bands indicated by the arrowheads) in lysates of Hela cells (- lane). Cells treated with MLF1IP / PBIP1 shRNA (+ lane) show no staining. MLF1 was detected using rabbit anti- MLF1 Antigen Affinity purified polyclonal antibody (Catalog # A07180) at 1:1000. Personal Communication, K.S. Lee, NCI, Bethesda, MD.

Additional image