A07180
antibody from Boster Biological Technology
Targeting: CENPU
CENP-50, CENP-U, KLIP1, MLF1IP, PBIP1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- A07180 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-MLF1 CENPU Antibody
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for PBIP1/CENPU detection. Host: Rabbit.Size: 100μL. Tested applications: IHC. Reactive species: Human. PBIP1/CENPU information: Molecular Weight: 47522 MW; Subcellular Localization: Cytoplasm. Nucleus. Chromosome, centromere, kinetochore. Localizes in the kinetochore domain of centromeres. Colocalizes with PLK1 at the interzone between the inner and the outer kinetochore plates; Tissue Specificity: Expressed at high levels in the testis, fetal liver, thymus, bone marrow and at lower levels in the lymph nodes, placenta, colon and spleen. Present in all cell lines examined, including B-cells, T-cells, epithelial cells and fibroblast cells. Expressed at high levels in glioblastoma cell lines.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100μL
- Concentration
- 0.88 mg/mL by UV absorbance at 280 nm
No comments: Submit comment
Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of MLF1 expression in detection of endogenous MLF1IP protein (a tier of four modified protein bands indicated by the arrowheads) in lysates of Hela cells (- lane). Cells treated with MLF1IP / PBIP1 shRNA (+ lane) show no staining. MLF1 was detected using rabbit anti- MLF1 Antigen Affinity purified polyclonal antibody (Catalog # A07180) at 1:1000. Personal Communication, K.S. Lee, NCI, Bethesda, MD.
- Additional image