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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB10333 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10333, RRID:AB_1710152
- Product name
- MLF1IP (phospho T78) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of MLF1IP.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Self-regulated Plk1 recruitment to kinetochores by the Plk1-PBIP1 interaction is critical for proper chromosome segregation.
Kang YH, Park JE, Yu LR, Soung NK, Yun SM, Bang JK, Seong YS, Yu H, Garfield S, Veenstra TD, Lee KS
Molecular cell 2006 Nov 3;24(3):409-22
Molecular cell 2006 Nov 3;24(3):409-22
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using MLF1IP (phospho T78) polyclonal antibody (Cat # PAB10333) shows detection of MLF1IP phosphorylated at Thr78.HeLa cells were co-infected with the indicated adenoviruses expressing GFP-tagged Plk1 or PBIP1.Blots were probed with the MLF1IP (phospho T78) polyclonal antibody (Cat # PAB10333), stripped, and then reprobed with anti-GFP antibody (Kang & Park, et al., 2006).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemistry of MLF1IP (phospho T78) polyclonal antibody (Cat # PAB10333) was used at 20 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.This image shows moderately positive staining of mitotic cells in colon adenocarcinoma at 60X.Tissue was formalin-fixed and paraffin embedded.The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.Personal Communi-cation, Tina Roush, Life Span Biosciences, Seattle, WA.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
