23306-1-AP

antibody from Invitrogen Antibodies

Targeting: WWTR1 DKFZp586I1419, TAZ

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

23306-1-AP

Provider

Invitrogen Antibodies

Product name

TAZ Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MNPASAPPP LPPPGQQVIH VTQDLDTDLE ALFNSVMNPK PSSWRKKILP ESFFKEPDSG SHSRQSSTDS SGGHPGPRLA GGAQHVRSHS SPASLQLGTG AGAAGSPAQQ HAHLRQQSYD VTDELPLPPG WEMTFTATGQ RYFLNHIEKI TTWQDPRKAM NQPLNHMNLH PAVSSTPVPQ RSMAVSQPNL VMNHQHQQQM APSTLSQQNH PTQNPPAGLM SMPNALTTQQ QQQQKLRLQR IQMERERIRM RQEELMRQEA ALCRQLPMEA ETLAPVQAAV NPPTMTPDMR SITNNSSDPF LNGGPYHSRE QSTDSGLGLG CYSVPTTPED FLSNVDEMDT GENAGQTPMN INPQQTRFPD FLDCLPGTNV DLGTLESEDL IPLFNDVESA LNKSEPFLTW L (1-400 aa encoded by BC014052)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.46 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of TAZ antibody (23306-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAZ transfected HeLa cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 23306-1-AP ( WWTR1,TAZ Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of WWTR1 antibody (TAZ antibody, 23306-1-AP, 1:1000) with LN229 cell lysate by Dr. Xiaobing He. (Lane 1, LN229 (GBM cell line) cell lysate. 90 ug; Lane 2, LN229 growth inhibition treatment. 90 ug).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 23306-1-AP ( WWTR1,TAZ Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 23306-1-AP ( WWTR1,TAZ Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-TAZ (IP:23306-1-AP, 4ug; Detection:23306-1-AP 1:300) with MDA-MB-453s cells lysate 2800ug.