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LearnPA1-46190
antibody from Invitrogen Antibodies
Targeting: WWTR1
DKFZp586I1419, TAZ
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA1-46190 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- WWTR1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Suggested positive control: 293 whole cell lysate, antigen standard for WWTR1 (transient overexpression lysate), HEK 293 lysate and kidney tissue lysate, human kidney protein.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot detection of PA1-46190 protein on HEK 293 cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of WWTR1 in HEK 293 cell lysate. Sample was incubated in WWTR1 polyclonal antibody (Product # PA1-46190).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of WWTR1 in 0.5 mg/mL HeLa lysate. Samples were incubated in WWTR1 polyclonal antibody (Product # PA1-46190). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of HL-60 (Lane 1), K-562 (Lane 2), MOLT-4 (Lane 3), U2 OS (Lane 4), HeLa (Lane 5), Panc1 (Lane 6) and A431 (Lane 7). The blot was probed with Anti-WWTR1 Polyclonal Antibody (Product # PA1-46190, 1:2000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 55kDa band corresponding to WWTR1 was seen in all cell lines tested except HL-60, K-562 and MOLT-4.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of WWTR1 in HepG2 cells. Samples were incubated in WWTR1 polyclonal antibody (Product # PA1-46190). Nuclei (Blue) are counterstained with Hoecsht 33258.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of WWTR1 in formalin fixed paraffin-embedded (FFPE) human kidney. Samples were incubated in WWTR1 polyclonal antibody (Product # PA1-46190) using a dilution of 1:500. Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear and cytoplasmic staining was observed. Staining was performed by Histowiz.
