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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- A300-608A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BTF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human BTF by western blot. Samples: Whole cell lysate (10 µg) from HEK293T, HeLa, A-549, MCF-7, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BTF antibody (Product # A300-608A lot 2) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse BTF by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BTF antibody (Product # A300-608A lot 2) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse BTF by western blot. Samples: Whole cell lysate (50 µg) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-BTF antibody A300-608A (lot A300-608A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human BTF by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-BTF antibody BL2521 (Cat. No. A300-608A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). BTF was also immunoprecipitated using rabbit anti-BTF antibodies BL2522 (Cat. No. A300-609A) and BL2523 (Cat. No. A300-610A) at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 3 seconds (A and B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human BTF by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BTF (Product # A300-608A; lot 2) used at a dilution of 1:1,000 (0.2 µg/mL). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human BTF by immunohistochemistry. Sample: FFPE section of human skin carcinoma. Antibody: Affinity purified rabbit anti-BTF (Cat. No. A300-608A) used at a dilution of 1:1,000 (0.2µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human BTF by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BTF antibody (Product # A300-608A lot 2) used for IP at 6 µg per reaction. BTF was also immunoprecipitated by a previous lot of this antibody (A300-608A lot 1) and another antibody against a different epitope of BTF (Product # A300-610A). For blotting immunoprecipitated BTF (Product # A300-608A) was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 1 second.
