Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 10221-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PDLIM7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: SFKVVLEGP APWGFRLQGG KDFNVPLSIS RLTPGGKAAQ AGVAVGDWVL SIDGENAGSL THIEAQNKIR ACGERLSLGL SRAQPVQSKP QKASAPAADP PRYTFAPSVS LNKTARPFGA PPPADSAPQQ NGQPLRPLVP DASKQRLMEN TEDWRPRPGT GQSRSFRILA HLTGTEFMQD PDEEHLKKSS QVPRTEAPAP ASSTPQEPWP GPTAPSPTSR PPWAVDPAFA ERYAPDKTST VLTRHSQPAT PTPLQSRTSI VQAAAGGVPG GGSNNGKTPV CHQCHKVI (3-289 aa encoded by BC001093)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.4 mg/mL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10221-1-AP (PDLIM7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 10221-1-AP (PDLIM7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of PDLIM7 antibody (10221-1-AP; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDLIM7 transfected HeLa cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF-7 cells were subjected to SDS PAGE followed by western blot with 10221-1-AP (PDLIM7 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed SKOV-3 cells using 10221-1-AP (PDLIM7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human ovary tumor using 10221-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 25x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of PDLIM7 antibody (10221-1-AP for IP and Detection) with HeLa cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-PDLIM7,LMP1 (IP:10221-1-AP, 4 ug; Detection:10221-1-AP 1:300) with MCF-7 cells lysate 2000 ug.
